Molecular elucidation of race-cultivar specificity in rice blast disease by using the rice mutants.

利用水稻突变体对稻瘟病的种族-品种特异性进行分子阐明。

基本信息

批准号：
09660050
负责人：
ARASE Sakae
金额：
$ 2.11万
依托单位：
SHIMANE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

In this study, the production of race-specific elicitors by M.grisea relating to expression of resistance based on gene-for-gene theory in rice blast disease was investigated. Rice leaf-sheaths of cv.Sekiguchi-asahi which had been pre-inoculated with a compatible race of M.grisea were inoculated with a nonpathogen or an incompatible race in the presence of the spore germination fluids from incompatible race of M.grisea. When the penetration and hyphal growth of post-inoculated fungi in leaf-sheath cells were investigated 36 hr after post-inoculation, there is a large difference in infection behaviors of fungi among spore germination fluids. In the case of the spore germination fluid (sgf) from incompatible races of M.grisea germinated on rice leaf-sheaths for 24 hr, penetration and hyphal growth of A.alternata or incompatible race were significantly inhibited. The sgf from incompatible race induced inaccessibility in healthy leaf-sheaths without pre-inoculation with the compatible race of M.grisea. Further, such induced-inaccessibility was also observed by treatment with the sgf from the incompatible race of M.grisea germinated on the susceptible cultivar Mohoto. In the case of spore germination fluid (SGF) from incompatible race of M. grisea germinated on plastic plates, however, above mentioned induced-inaccessibility phenomena were not observed. The elicitor activity of the sgf was not destructed by heat treatment at 120 C for 20 min. From gel filtration and dialysis tests, it was suggested that molecular weight of the elicitors were smaller than 5,000. From extraction test with organic solvents, it was demonstrated that the elicitors were water-soluble. These results indicate that M.grisea spores produce the elicitors concerning to induced inaccessibility only on host tissues regardless of the compatibility between cultivar and fungal race.

在这项研究中，研究了基于基因 - 基因理论在水稻爆炸疾病中基于基因理论的耐药性而产生的种族特异性引起者。在不兼容的M.Grisea种族的孢子发芽液的存在下，已接种了与M.Grisea兼容种族的CV.Sekiguchi-Asahi的米叶店。当接种后36小时，研究了接种后真菌在叶鞘细胞中的渗透和菌丝生长时，在孢子发芽液中真菌的感染行为差异很大。如果是在米叶羊皮上发芽的不兼容种族的孢子发芽液（SGF）24小时，则明显抑制A.Alternata或不兼容种族的穿透和菌丝生长。来自不兼容种族的SGF引起了健康叶鞘的无法获得，而没有与M.Grisea兼容种族预接种。此外，还通过用易感品种莫霍托（Mohoto）发芽的M.grisea种族的SGF治疗SGF来观察到这种诱发的无障碍性。在孢子发芽液（SGF）的情况下，从塑料板上发芽的grise菌种族不兼容，但未观察到上述诱导的不可接受现象。 SGF的启发性活性在120℃下不会被热处理破坏20分钟。从凝胶过滤和透析测试中，提示引起剂的分子量小于5,000。从有机溶剂的提取试验中，可以证明引起剂是水溶性的。这些结果表明，麦芽孢子孢子产生有关引起的诱导者，仅在宿主组织上引起无法访问，而不管品种和真菌种族之间的兼容性如何。