FUNCTION OF PLANT GROWTH PROMOTING FUNGI AND THEIR CONTROL OF SOIL-BORNE DISEASES

植物促生真菌的作用及其对土传病害的防治

• 批准号: 09660044
• 负责人: HYAKUMACHI Mitsuro
• 金额: $ 1.79万
• 依托单位: GIFU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09660044/
• 关键词: PLANT GROWTH PROMOTING FUNGI; INDUCED RESISTANCE; ANTHRACNOSE; BACTERIAL ANGULARLEAF SPOT; FUSARIUM WILT; SOIL-BORNE DISEASES; CUCUMBER; PLANT GROWTH PROMOTING FUNGI; INDUCED RESISTANCE; ANTHRACNOSE; BACTERIAL ANGULARLEAF SPOT; FUSARIUM WILT; SOIL-BORNE DISEASES; CUCUMBER

Plant growth promoting fungi (PGPF) exhibited the ability of plant growth promotion as well as disease suppression. Some PGPF isolates were also found capable of inducing systemic protection in cucumber against Colletotrichum orbiculare, the anthracnose pathogen. Cucumber plants even treated with culture filatrates (CFs) of PGPF induced a systemic resistance against this disease. Roots treated with CFs induced lignification at the points of attemped penetration by the pathogen indicating that elicitor substance(s) existed in the CFs. The elicitor activity of CFs was evaluated by the chemiluminescence assay using cucumber fruit disks. The emission of chemiluminescence from cucumber fruit disks treated with CFs was determined using ARGUS-50. The CFs of PGPF elicited conspicuous superoxide generation. The chemiluminescence activity of the CF of Penicillium GP17-2 was extremely high, and its intensity was almost 100 X that of other isolates. The activity was not lost after treatment with protease or autoclaving or after removal of lipid. The dialyzed CF fraction containing substances of MW.more than 2,000,000 was highly effective. This fraction could induce conspicuous lignification in the epidermal tissues of cucumber hypocotyl. Both lignification and superoxide generation are thought to be intimately related with the expression of induced resistance.

促进真菌（PGPF）的植物生长表现出植物生长促进以及抑制疾病的能力。还发现一些PGPF分离株能够在黄瓜中诱导全身性保护抗炭疽病病原体的Colletotrichum Orbiculare。 PGPF的培养物（CFS）甚至用培养物治疗的黄瓜植物诱导了对该疾病的全身耐药性。用CFS治疗的根部通过病原体在穿透的点处处理的根部，表明CFS中存在诱发剂物质。通过使用黄瓜水果盘进行化学发光测定法评估CFS的启发性活性。使用Argus-50确定了用CFS处理的黄瓜水果盘的化学发光。 PGPF的CFS引起了明显的超氧化物的产生。 gp17-2的CF的化学发光活性非常高，其强度几乎是其他分离株的100 x。用蛋白酶或高压灭菌或去除脂质后，该活性不会损失。含有MW的物质的透析CF分数超过200万种。该部分可能会诱导黄瓜下胚轴表皮组织中的明显木质化。木质化和超氧化物的产生都与诱导的抗性表达密切相关。