Correlation between the expression of WAF1 and the tumor growth in human pancreatic cancer

WAF1表达与人胰腺癌肿瘤生长的相关性

• 批准号: 09671342
• 负责人: HARADA Nobuhiko
• 金额: $ 1.92万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671342/
• 关键词: pancreatic cancer; p53; p21; WAF1; prognosis; p21WAF1; アポトーシス; 膵癌細胞株

Sixty-two resected specimens of human ductal adenocarcinoma of the pancreas were investigated on p53 and p21WAF1 immunohistochemically and genetically by RT-PCR.Also correlation between the expression of p53 or p21WAF1 and the prognosis of the patients were studied. Twenty frozen specimens of pancreatic cancer tissue and 6 pancreatic cancer cell lines were checked up by Westernblot and RT-PCR.In immunohistochemistry, 37% of the patients showed p53 positive and 48% showed p21WAF1 positive. Wild-type p53 significantly correlated not only with p21WAF1 protein expression (p <0.03) but also with WAF1 mRNA expression (p <0.005). The mean survival time was 16 months in the patients with p53(-), 10.5 months in p53(+), 12 months in p2l(-) and 15 months in p2l(+). Both WAF1 protein and mRNA were detected only in the pancreatic cancer cell lines with wild-type p53 but not in those with mutant-p53. In pancreatic cancer, wild-type p53 corresponds significantly to the WAF1 expression and this correlations more closely on the mRNA level than on the protein level.Our data suggest a tendency for the tumors expressing mutant-p53 and not expressing p21WAF1 to grow faster.

对62例人胰腺导管腺癌切除标本进行p53和p21WAF1免疫组化和RT-PCR遗传学检测，并研究p53和p21WAF1表达与患者预后的相关性。采用Westernblot和RT-PCR检测20份冷冻胰腺癌组织标本和6个胰腺癌细胞系。免疫组化结果显示，37%的患者p53阳性，48%的患者p21WAF1阳性。野生型p53不仅与p21WAF1蛋白表达显着相关(p<0.03)，而且与WAF1mRNA表达显着相关(p<0.005)。 p53(-)患者的平均生存时间为16个月，p53(+)患者的平均生存时间为10.5个月，p2l(-)患者的平均生存时间为12个月，p2l(+)患者的平均生存时间为15个月。 WAF1 蛋白和 mRNA 仅在具有野生型 p53 的胰腺癌细胞系中检测到，而在具有突变型 p53 的胰腺癌细胞系中未检测到。在胰腺癌中，野生型p53与WAF1表达显着对应，并且这种相关性在mRNA水平上比在蛋白质水平上更密切。我们的数据表明表达突变型p53而不表达p21WAF1的肿瘤有生长更快的趋势。

项目成果

N,HARADA.: "Nuclear accumulation of p53 corretates significantly with clinical features and inversely with the expression of p21WAF1" British Journal of Cancer. 76(3). 299-305 (1997)

N,HARADA.：“p53 的核积累与临床特征显着相关，与 p21WAF1 的表达成反比”《英国癌症杂志》。