Correlation between the expression of WAF1 and the tumor growth in human pancreatic cancer

WAF1表达与人胰腺癌肿瘤生长的相关性

• 批准号: 09671342
• 负责人: HARADA Nobuhiko
• 金额: $ 1.92万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671342/
• 关键词: pancreatic cancer; p53; p21; WAF1; prognosis; p21WAF1; アポトーシス; 膵癌細胞株; pancreatic cancer; p53; p21; WAF1; prognosis; p21WAF1; アポトーシス; 膵癌細胞株

Sixty-two resected specimens of human ductal adenocarcinoma of the pancreas were investigated on p53 and p21WAF1 immunohistochemically and genetically by RT-PCR.Also correlation between the expression of p53 or p21WAF1 and the prognosis of the patients were studied. Twenty frozen specimens of pancreatic cancer tissue and 6 pancreatic cancer cell lines were checked up by Westernblot and RT-PCR.In immunohistochemistry, 37% of the patients showed p53 positive and 48% showed p21WAF1 positive. Wild-type p53 significantly correlated not only with p21WAF1 protein expression (p <0.03) but also with WAF1 mRNA expression (p <0.005). The mean survival time was 16 months in the patients with p53(-), 10.5 months in p53(+), 12 months in p2l(-) and 15 months in p2l(+). Both WAF1 protein and mRNA were detected only in the pancreatic cancer cell lines with wild-type p53 but not in those with mutant-p53. In pancreatic cancer, wild-type p53 corresponds significantly to the WAF1 expression and this correlations more closely on the mRNA level than on the protein level.Our data suggest a tendency for the tumors expressing mutant-p53 and not expressing p21WAF1 to grow faster.

研究了p53和p21WAF1免疫组织化学和遗传上的p21WAF1在p53或p21WAF1的表达与患者预后之间的相关性，研究了62个胰腺的人类导管腺癌标本。 Westernblot和Rt-Pcr.in免疫组织化学检查了20个冷冻标本，并检查了6个胰腺癌细胞系，37％的患者显示p53阳性，48％的患者显示p21WAF1阳性。野生型p53不仅与P21WAF1蛋白表达（P <0.03）显着相关，而且与WAF1 mRNA表达（P <0.005）相关。 p53（ - ）患者的平均生存时间为16个月，p53（+）为10.5个月，在P2L（ - ）中为12个月，P2L（+）为15个月。仅在具有野生型p53的胰腺癌细胞系中检测到WAF1蛋白和mRNA，但在患有突变体p53的胰型p53中均未检测到。在胰腺癌中，野生型p53与WAF1表达显着相对应，并且在mRNA水平上的相关性比蛋白质水平更接近。