Development and application of tooth surface modifier having plaque-controlling ability.

具有牙菌斑控制能力的牙面改性剂的研制及应用

基本信息

批准号：
09671970
负责人：
TERANAKA Toshio
金额：
$ 2.11万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 2000
项目状态：
已结题

项目摘要

The cause of the plaque accumulation is attributed to the attachment of microorganisms to the solid surface brought about by physicochemical interactions. Silane coupling agents containing fluorocarbon chains have several characteristics such as water and oil repellency, prevention of contamination, high lubricity, and thermal and chemical stability. Yoshino et al. synthesized two dental silane coupling agents named 1H, 1H, 2H, 2H-henicosafluorododecyltriisocyanatosilane (10F2S-3I) and 1H, 1H, 2H, 2H-henicosafluorododecyltrimethoxysilane (10F2S-3M). The surface modification of resin composite with 10F2S-3I or 10F2S-3M has shown a preventive effect on the initial adherence of bacteria to composite surface. In the present experiment, the surface modification efficacy of 10F2S-3I was evaluated on the enamel surface.The bovine teeth specimens were cut and smoothly finished. The specimens were divided into three groups : 1) control group without treatment, 2) surface modified group (MG) : a … More pplied 30.0mmol/l of 10F2S-3M or dodecyltrimethoxysilane (10H2S-3M), or 3.0mmol/l of 10F2S-3I, and 3) pretreated and surface modified group (PMG) : applied each silane after etching with phosphoric acid (PMG). Surface free energy, the amount of calcium dissolution, accumulation of Streptococcus mutans in vitro, and accumulation of plaque in vivo were evaluated in each group of specimens. The hydroxyapatite powder (HAP) and bovine enamel powder (BEP) were also treated with each silane, and amount of adsorbed phosvitin was determined by gel permeation chromatography. The data were analyzed statistically using ANOVA and Fisher PLSD at p<0.05.The surface free energy of MG and PMG modified with 10F2S-3I or 10F2S-3M were significantly lower compared with control. The amount of phosvitin adsorption onto both HAP and BEP treated with 10F2S-3I or 10F2S-3M were significantly less than these of controls. The amount of calcium dissolution from MG and PMG after 60 minutes was significantly less than those of control. The total amount of S.mutans accumulated on MG and PMG modified with 10F2S-3I were significantly less than that of control. The total amounts of plaque accumulated on MG and PMG modified with 10F2S-3I using intra oral plaque accumulation test appliance were significantly less than compared with control after 7 days. There were no significant difference was found between the MG and PMG on the amount of plaque accumulation.From the results obtained in this study, it was suggested that the surface modifier 10F2S-3I significantly reduce plaque adherence to enamel. In conclusion, the use of 10F2S-3I may have clinical contribution to maintenance of oral health. Less

菌斑堆积的原因是微生物通过物理化学相互作用而附着在固体表面，含有氟碳链的硅烷偶联剂具有拒水拒油、防污染、高润滑性、耐热性和化学性等特性。 Yoshino 等人合成了两种牙科硅烷偶联剂，命名为 1H、1H、2H、2H-二十二碳六氟十二烷基三异氰酸酯硅烷。 (10F2S-3I)和1H、1H、2H、2H-二十一氟十二烷基三甲氧基硅烷(10F2S-3M) 用10F2S-3I或10F2S-3M对树脂复合材料进行表面改性，对细菌在复合材料表面的初始粘附显示出预防作用。本实验评估了10F2S-3I对牙釉质的表面改性效果。将牛牙齿标本切割并光滑地抛光，将标本分为三组：1）未处理的对照组，2）表面改性组（MG）：施加30.0mmol/l的10F2S-3M或十二烷基三甲氧基硅烷。 (10H2S-3M)，或3.0mmol/l的10F2S-3I，和3)预处理和表面改性基团(PMG)：对各组样品进行磷酸蚀刻(PMG)后的表面自由能、钙溶解量、体外变形链球菌的积累以及体内斑块的积累。还用每种硅烷处理牛釉质粉末（BEP），并通过凝胶渗透色谱法测定吸附的卵黄高磷蛋白的量。使用 ANOVA 和 Fisher PLSD 进行分析，p < 0.05。与对照相比，用 10F2S-3I 或 10F2S-3M 修饰的 MG 和 PMG 的表面自由能显着降低。用 10F2S 处理的 HAP 和 BEP 上的卵黄高磷蛋白吸附量 -。 3I或10F2S-3M后MG和PMG的钙溶出量明显少于对照。 60分钟后，在用10F2S-3I修饰的MG和PMG上累积的变形链球菌总量显着少于在用10F2S-3I修饰的MG和PMG上累积的噬菌斑总量。 7天后，使用口腔内菌斑累积测试装置与对照相比，MG和PMG的菌斑累积量没有显着差异。从本研究获得的结果来看，MG和PMG之间的菌斑累积量没有显着差异。有人认为表面改性剂 10F2S-3I 可以显着减少牙菌斑对牙釉质的粘附。总之，使用 10F2S-3I 可能对维护口腔健康有临床贡献。