Tertiary structure of transcriptional co-activators.

转录共激活子的三级结构。

基本信息

批准号：
09680652
负责人：
SHIRAKAWA Masahiro
金额：
$ 2.05万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Bombyx mori and human MBFlTertiary structures of the core domains of Born byx mori and human MBFI were determined by means of multi-dimensional multi-nuclear NMR.The core domains are capable of binding to TBP.Both of them consists of four a helices and the connecting loops. By mutation analyses, residues indispensable for the transactivations have been identified.The central domain of human repair factor XPAThe solution structure of the central domain of the human nucleotide excision repair (NER) protein XPA, which is responsible for the binding to damaged DNA and replication protein A (RPA), was determined by NMR spectroscopy. The central domain consists of a zinc-containing subdomain and a carboxyl-terminal subdomain. The zinc-containing subdomain has a compact globular structure and is distinct from the zinc-fingers found in transcription factors. The carboxyl-terminal subdomain folds into a novel alpha / beta structure with a positively charged superficial cleft, From the NMR spectra of the complexes, DNA and RPA binding surfaces are suggested.The complex of hDLG PDZ domain between the C-terminal of APCTertiary structure of the complex between the PDZ2 domain of human tumor suppressor hDLG and the C-terminal peptide was determined by multi-dimensional multi-nuclear NMR.The PDZ2 folds into an a /beta structure with a cleft formed between a beta sheet and an a helix. The bound C-terminal peptide of APC was found to be located in the cleft, making hydrophobic and electric interactions with the PDZ2 domain.F.coli ArcBStructure of the phosphotransfer domain of E.ccli sensor kinase ArcB was determined by multi-dimensional multi-nuclear NMR.It folds into an a structure with five helices. Analyses of the dynamic properties of the domain by means of measuring 15N relaxation rates revealed that the are that contains the active histidine exhibited a characteristic dynamic property.

家蚕和人MBFl通过多维多核NMR测定了家蚕和人MBFI核心结构域的三级结构。核心结构域能够与TBP结合。两者均由四个a螺旋组成，连接环路。通过突变分析，已鉴定出反式激活所必需的残基。人类修复因子XPA的中心结构域人类核苷酸切除修复（NER）蛋白XPA的中心结构域的溶液结构，负责与受损DNA的结合和复制蛋白质A (RPA)，通过核磁共振波谱法测定。中心结构域由含锌亚结构域和羧基末端亚结构域组成。含锌子结构域具有紧凑的球状结构，与转录因子中的锌指不同。羧基末端亚结构域折叠成具有带正电荷的表面裂口的新型α/β结构，从复合物的NMR谱，提示DNA和RPA结合表面。 APC的C端之间的hDLG PDZ结构域的复合物三级结构通过多维多核NMR测定人肿瘤抑制因子hDLG的PDZ2结构域与C端肽之间的复合物的结构。PDZ2折叠成α/β结构β折叠和α螺旋之间形成的裂缝。发现APC结合的C端肽位于裂口处，与PDZ2结构域产生疏水性和电性相互作用。F.coli ArcB通过多维多维测定确定了E.ccli传感器激酶ArcB的磷酸转移结构域的结构。核磁共振。它折叠成具有五个螺旋的结构。通过测量 15N 弛豫率对结构域的动态特性进行分析表明，含有活性组氨酸的结构域表现出特有的动态特性。