Ca^<2+> imaging of the slug nervous system underlying the odor-taste associative learning

Ca^<2> 气味-味觉关联学习背后的蛞蝓神经系统成像

基本信息

批准号：
09680646
负责人：
KAWAHARA Shigenori
金额：
$ 2.11万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680646/
关键词：
Slug Invertebrate Learning Synchronization Potential Oscillation Ca^<2+>-sensitive dye Optical Recording カルシウム

项目摘要

The procerebrum, a central olfactory organ of The terrestrial mollusc Limax marginatus, is clearly compartmentalized to a cell mass consisting of cell bodies of the local interneurons and neuropils where neurites of intrinsic and extrinsic neurons are interconnected. To clarify the mechanisms of odor information processing and odor-taste associative learning, responses of The procerebrum to olfactory input should be analyzed using an optical recording technique. For this purpose, it is most desirable to obtain signals only from the cell mass in its posterior view without interference by the signals from the underlying neuropils which contain those of neurites of extrinsic neurons as well. In the present study, fluorescent Ca^<2+> indicator dyes were used to stain selectively The cell mass for Their incorporation into cytoplasm of the cell soma, while potential-sensitive dyes were incorporated into the cell membranes to record predominantly electroactivities of the neuropils. A differential imaging system, which has a wide dynamic range virtually, well extracted signals of intracellular calcium from large background fluorescence which in general accompanied tissue-staining with acetoxymethyl esters of Ca^<2+> indicator dyes. Ca^<2+> signals conveying information on the neural activity of the CM exhibited synchronous oscillation, wave propagation, and responses to the excitation of the olfactory nerve. The present study indicates that optical recording with Ca^<2+> indicator dyes provides a useful means to selectively observe neural activities of The cell mass of the procerebrum.

前大脑是陆生软体动物边缘蜥蜴的中央嗅觉器官，它被清楚地划分为由局部中间神经元和神经毡的细胞体组成的细胞团，其中内在和外在神经元的神经突相互连接。为了阐明气味信息处理和气味-味觉关联学习的机制，应使用光学记录技术来分析前大脑对嗅觉输入的反应。为此目的，最理想的是仅从其后视图中的细胞团获得信号，而不受来自下面的神经纤维的信号的干扰，所述神经纤维也包含外在神经元的神经突的信号。在本研究中，荧光Ca^2+指示剂染料用于选择性染色细胞团，使其掺入细胞体的细胞质中，而电位敏感染料则掺入细胞膜中，主要记录神经纤维的电活性。。差分成像系统实际上具有宽的动态范围，可以从通常伴随着Ca 2+ 指示剂染料的乙酰氧基甲基酯的组织染色的大背景荧光中很好地提取细胞内钙的信号。传达CM神经活动信息的Ca 2+ 信号表现出同步振荡、波传播和对嗅觉神经兴奋的反应。本研究表明，用Ca 2+ 指示剂染料进行光学记录提供了选择性观察前大脑细胞团的神经活动的有用手段。