Analysis of the cytoplasmic radiation responses by using X-ray microbeam irradiation techniques

利用 X 射线微束辐照技术分析细胞质辐射响应

• 批准号: 23710076
• 负责人: MAEDA Munetoshi
• 金额: $ 2.83万
• 依托单位: The Wakasa Wan Energy Research Center (2012-2013)Central Research Institute of Electric Power Industry (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23710076/
• 关键词: 生物影響; マイクロビーム; 細胞局所照射; 細胞質の放射線応答; バイスタンダー応答; 突然変異; 選択的細胞死; 一酸化窒素 (NO); 突然変異誘発; 突然変異抑制; 一酸化窒素（NO); マイクロコロニー法; 蛍光抗体法; 修復関連タンパク質; 突然変異誘発頻度; 一酸化窒素

Microbeam cell irradiation system is a powerful tool in the elucidation of the mechanisms underlying biological responses to low-dose radiations. In this study, we analyzed the relationship between irradiation domain in the cells and the cellular responses with new X-ray microbeam irradiation techniques. Our results clearly demonstrated that the signaling process of DNA repair responses were modified via cytoplasmic radiation responses. Furthermore, we found that radiation-induced bystander responses can enhance selective cell killing of genetically unstable cells in the bystander cell population and that this selective cell death may act as a protective mechanism that competes with increases in non-lethal and potentially carcinogenic damages, e.g. mutations. Intracellular communication between the nucleus and cytoplasm plays an important role in determining cell death in the low-dose region. Our findings may provide important information to evaluate the risk of low doses of radiation.

微束细胞辐照系统是阐明低剂量辐射生物反应机制的有力工具。在本研究中，我们分析了细胞内的照射域与新的 X 射线微束照射技术的细胞反应之间的关系。我们的结果清楚地表明 DNA 修复反应的信号传导过程是通过细胞质辐射反应改变的。此外，我们发现辐射诱导的旁观者反应可以增强旁观者细胞群中遗传不稳定细胞的选择性细胞杀伤，并且这种选择性细胞死亡可能作为一种保护机制，与非致命和潜在致癌损伤的增加相竞争，例如，突变。细胞核和细胞质之间的细胞内通讯在决定低剂量区域的细胞死亡中起着重要作用。我们的研究结果可能为评估低剂量辐射的风险提供重要信息。

项目成果

Reduction of mutation in bystander cells caused by nitric oxide-mediated bystander cell death

减少一氧化氮介导的旁观细胞死亡引起的旁观细胞突变

• 发表时间: 2013
• 作者: Munetoshi MAEDA; Katsumi KOBAYASHI; Hideki MATSUMOTO; Noriko USAMI; Masanori TOMITA
• 通讯作者: Masanori TOMITA

Reduction of spontaneous mutagenesis by bystander cell death in V79 cells

V79 细胞中旁观者细胞死亡减少自发突变

• 发表时间: 2012
• 作者: Munetoshi MAEDA; Katsumi KOBAYASHI; Hideki MATSUMOTO; Noriko USAMI; Masanori TOMITA
• 通讯作者: Masanori TOMITA

放射光単色 X線マイクロビーム細胞照射装置を用いた細胞応答の解析

使用同步加速器单色X射线微束细胞照射设备分析细胞反应

• 发表时间: 2011
• 作者: 前田 宗利

放射線照射後の細胞死（Cell death after irradiation: how, when and why cells die)

辐射后细胞死亡：细胞如何、何时以及为何死亡

• 作者: 前田 宗利

X-ray-induced Nitric Oxide-mediated Bystander Cell Death Suppresses Spontaneous Mutagenesis in V79 Cells

X 射线诱导的一氧化氮介导的旁观细胞死亡抑制 V79 细胞的自发突变

• 发表时间: 2014
• 作者: Munetoshi MAEDA; Katsumi KOBAYASHI; Hideki MATSUMOTO; Noriko USAMI; Masanori TOMITA
• 通讯作者: Masanori TOMITA