Biological Solid-State NMR for Challenging Samples - Sensitivity Enhancement using Visible Light

用于挑战性样品的生物固态 NMR - 使用可见光增强灵敏度

• 批准号: 23770114
• 负责人: MATSUKI Yoh
• 金额: $ 2.91万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770114/
• 关键词: 固体NMR; 感度向上; 光化学誘起動的核分極; 固体核磁気共鳴法; 光励起核偏極; 高感度化; 可視光レーザー; 動的核分極; 高感度固体NMR

Instruments and fundamental techniques have been developed toward establishing a general way for enhancing sensitivity of solid-state NMR via nuclear hyperpolarization based on photo excited dye molecules: photo-chemically induced dynamic nuclear polarization (CIDNP). Using newly developed photo-sensitizer system using flavin derivatives and imidazole embedded in a micelle, enhancement of net nuclear polarization via photo-CIDNP has been observed. It was also found that paramagnetic relaxation enhancement (PRE) effect of the photo-excited dye molecules significantly accelerates the longitudinal relaxation rate of bulk nuclear polarization, enabling rapid signal accumulation or unit-time sensitivity enhancement. The above methods based on light-excited dye overcome a major drawbacks of conventional DNP and PRE techniques: paramagnetic signal broadening, because the paramagnetic species can be quenched by turning the light illumination off during signal acquisition.

仪器和基础技术的发展旨在建立一种通过基于光激发染料分子的核超极化来增强固态 NMR 灵敏度的通用方法：光化学诱导动态核极化 (CIDNP)。使用新开发的光敏剂系统，将黄素衍生物和咪唑嵌入胶束中，观察到通过光 CIDNP 增强净核极化。研究还发现，光激发染料分子的顺磁弛豫增强（PRE）效应显着加速了体核极化的纵向弛豫速率，从而实现了快速信号积累或单位时间灵敏度增强。上述基于光激发染料的方法克服了传统DNP和PRE技术的主要缺点：顺磁信号展宽，因为顺磁物质可以通过在信号采集期间关闭光照明来淬灭。

项目成果

Boosting Protein Dynamics Studies using High-Sensitivity Non-Uniform Sampling (NUS) NMR

使用高灵敏度非均匀采样 (NUS) NMR 促进蛋白质动力学研究

• 发表时间: 2012
• 作者: 松木 陽

Simulated Annealing Spectral Fitting Approach to Signal Assignment and Secondary Structure Analysis of Beta-2 Microglobulin (b2m) in Fibril Form

原纤维形式 Beta-2 微球蛋白 (b2m) 的信号分配和二级结构分析的模拟退火光谱拟合方法

• 发表时间: 2013
• 作者: Yoh Matsuki

NMR Signal Enhancement by Solid-State Laser Induced Paramagnetic Relaxation

固态激光诱导顺磁弛豫增强 NMR 信号

• 发表时间: 2013
• 作者: Y. Matsuki

光を使った固体NMRの汎用高感度化法の開発

开发利用光进行固态核磁共振的通用高灵敏度方法

• 发表时间: 2013
• 作者: 松木陽