Development of the novel high precision low labor load gene-diagnosis system towards realization of tailor-made medical care

开发新型高精度低劳动负荷基因诊断系统，实现个性化医疗

• 批准号: 23550092
• 负责人: TAKAHASHI Toru
• 金额: $ 3.33万
• 依托单位: University of Fukui
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23550092/
• 关键词: 一塩基多型; キャピラリー電気泳動; 一塩基多型検出; ジェノタイピング; SNP検出; 抽出ゲノム; PCR; SNP; 一塩基変異一本鎖DNA; 塩基; プロトン解離; 分離; 検出; 判定法

A multiple detection method for single nucleotide polymorphism (SNP) based on the CE separation technique for single base substituted sequence isomers of single stranded DNA was proposed. In the CE separation method where an acidic aqueous urea solution was employed as an electrophoretic buffer solution, the separation of single base substituted sequence isomers of single stranded DNA was achieved according to the difference of the proton dissociation properties of each DNA bases at acidic condition. A clear criterion for determining of existence or nonexistence of SNP by the peak pattern of the electropherogram allows high reliability of the proposed method. The proposed method can be applied to all substitution patterns with identical condition. The proposed method was successfully applied to the SNP detection and genotyping for extracted human gene fragment sample including ALDH2 gene region.

提出了一种基于单链DNA单碱基取代序列异构体CE分离技术的单核苷酸多态性(SNP)多重检测方法。 CE分离方法采用酸性尿素水溶液作为电泳缓冲液，根据各DNA碱基在酸性条件下质子解离性质的差异，实现单链DNA单碱基取代序列异构体的分离。通过电泳图的峰值模式确定 SNP 存在或不存在的明确标准使得所提出的方法具有高可靠性。所提出的方法可以应用于具有相同条件的所有替换模式。该方法成功应用于提取的人类基因片段样本（包括ALDH2基因区域）的SNP检测和基因分型。

项目成果

Capillary Electrophoretic Reactor and Microchip Capillary Electrophoretic Reactor: Dissociation Kinetic Analysis Method for "Complexes" Using Capillary Electrophoretic Separation Process, in C. D. Garcia, K. Chumbimuni Torres, and E. Carrilho (Eds.), Fund

毛细管电泳反应器和微芯片毛细管电泳反应器：使用毛细管电泳分离过程的“复合物”解离动力学分析方法，C. D. Garcia、K. Chumbimuni Torres 和 E. Carrilho（Eds.），基金

• 发表时间: 2012
• 作者: T. Takahashi; N. Iki
• 通讯作者: N. Iki

Dissociation Kinetic Analysis of Ce(III) complex with Quin2 by Microchip Capillary Electrophoretic Reactor

利用微芯片毛细管电泳反应器分析 Ce(III) 与 Quin2 配合物的解离动力学

• 发表时间: 2013
• 作者: Kei;Toru TAKAHASHI
• 通讯作者: Toru TAKAHASHI

in Fundamental Concepts, Practical Applications, and Limitations of Capillary Electrophoresis and Microchip Capillary Electrophoresis, C. D. Garcia, K. Chumbimuni Torres, and E. Carrilho (Eds.), Capillary Electrophoretic Reactor and Microchip Capillary El

毛细管电泳和微芯片毛细管电泳的基本概念、实际应用和局限性，C. D. Garcia、K. Chumbimuni Torres 和 E. Carrilho（编辑），毛细管电泳反应器和微芯片毛细管电泳

• 发表时间: 2013
• 作者: T. Takahashi; N. Iki
• 通讯作者: N. Iki

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

非离子聚合物混合溶液作为涂层剂和分离介质的动态涂层毛细管电泳分离腐植酸

• 发表时间: 2013
• 作者: T. Takahashi; J. Kawana; Y. Tamura; H. Hoshino
• 通讯作者: H. Hoshino

ハイブリダイズによらない一塩基多型検出およびジェノタイピング：一本鎖DNAのキャピラリー電気泳動分離による一塩基変異の直接識別

无需杂交的单核苷酸多态性检测和基因分型：通过单链DNA毛细管电泳分离直接鉴定单核苷酸突变

• 发表时间: 2013
• 作者: 高橋 透;深川 真有;櫻井 隆郎;星野 仁
• 通讯作者: 星野 仁