Multi-omics study to elucidate the molecular mechanism of anhydrobiosis in tardigrade Ramazzottius varieornatus

多组学研究阐明缓步动物 Ramazzottius varieornatus 脱水的分子机制

• 批准号: 22681029
• 负责人: ARAKAWA Kazuharu
• 金额: $ 12.98万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22681029/
• 关键词: メタボローム; システム生物学; プロテオーム; 乾眠; クマムシ; クリプトビオシス; マルチオミクス; バイオインフォマティクス; トランスクリプトーム; 乾燥耐性; ヨコヅナクマムシ; Hypsibius dujardini; CE-MS; LC-MS; 極限環境

Limno-terrestrial tardigrades can withstand extreme environments and almost complete desiccation through a mechanism called anhydrobiosis, but the its molecular mechanism is yet to be explored. To this end, we have conducted a comprehensive multi-omics analysis using the tardigrade Ramazzottius varieornatus, which is a potential model species for anhydrobiosis. In order to analyze the metabolic changes in the active and dehydrated states, we measured the metabolome in both conditions using two types of high-throughput mass spectrometry (MS) systems, CE-TOFMS and LC-TOFMS. Moreover, phospho-proteome was also analyzed to observed the metabolic regulations. As a result, while changes in gene expression profiles are limited in between active and tun states, dynamic changes were observed in the metabolism of this species in response to desiccation. Changes in the metabolic profiles suggests complex intracellular responses to oxidative and osmotic stress.

湖沼陆生缓步动物可以通过一种称为脱水生物的机制承受极端环境和几乎完全干燥，但其分子机制仍有待探索。为此，我们使用缓步动物 Ramazzottius varieornatus 进行了全面的多组学分析，这是一种潜在的缺水生物模式物种。为了分析活性和脱水状态下的代谢变化，我们使用两种类型的高通量质谱（MS）系统CE-TOFMS和LC-TOFMS测量了两种条件下的代谢组。此外，还分析了磷酸蛋白质组以观察代谢调节。因此，虽然基因表达谱的变化仅限于活跃状态和 tun 状态之间，但在该物种的新陈代谢中观察到了响应干燥的动态变化。代谢特征的变化表明细胞内对氧化和渗透应激有复杂的反应。

项目成果

Metabolomics of tardigrade Ramazzottius varieornatus reveals dynamic metabolic response during anhydrobiosis

缓步动物 Ramazzottius varieornatus 的代谢组学揭示了脱水过程中的动态代谢反应

• 发表时间: 2012
• 作者: Kazuharu Arakawa; Ito T; Kunieda T; Horikawa D; Soga T; Tomita M
• 通讯作者: Tomita M

Metabolomics of tardigrade Ramazzottius varieornatus reveals dynamic metabolic response during anhydrobiosis

缓步动物 Ramazzottius varieornatus 的代谢组学揭示了脱水过程中的动态代谢反应

• 发表时间: 2012
• 作者: Kazuharu Arakawa; Ito T; Kunieda T; Horikawa D; Soga T; Tomita M
• 通讯作者: Tomita M

G-language Bookmarklet : a gateway for Semantic Web, Linked Data, and Web Services

G 语言书签：语义 Web、链接数据和 Web 服务的网关

• 发表时间: 2010
• 作者: Kazuharu Arakawa

Metabolomics of tardigrade Ramazzottius varieornatus reveals dynamic metabolic response during anhydrobiosis

缓步动物 Ramazzottius varieornatus 的代谢组学揭示了脱水过程中的动态代谢反应

• 作者: Arakawa K

ヨコヅナクマムシはどのようにして生と死のはざまを生きるか

黄色缓步动物如何在生与死之间生存？

• 作者: 荒川和晴