A New Salting-out Phenomenon of Water-soluble Polymer at Solid-liquid Interface

水溶性聚合物固液界面盐析的新现象

• 批准号: 22J14976
• 负责人: GONG HAO
• 金额: $ 0.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-22 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22J14976/
• 关键词: LLPS; Separation; Solid-liquid Interface; DNA; PEGylation; Rapid; Salt; LLPS; Separation; Solid-liquid Interface; DNA; PEGylation; Rapid; Salt

The research project on new salting-out phenomena of water-soluble polymers at the solid-liquid interface is currently in progress and is on track to meet the established goals and objectives. Here, I discovered that PEGs with the same molecular weight but distinct termini can be instantly separated when an aqueous solution of their mixture with a very high salt-to-PEG weight ratio (~10^6) is drop-cast on a glass plate. A mixture of four polymers can rapidly be separated. Even when their termini are structurally analogous to each other with only a two carbon-atom and hydrogen-atom difference, they can be rapidly separated. I have completed the important stages of the project, including literature review, research design, and data collection. The data collected has been analyzed using appropriate scientific means, and the findings have been interpreted.Currently, we are working on resubmitting the research manuscript to high-impact journal, Nature, which will include a detailed analysis of the findings, conclusions, and recommendations for future research. Extending work on this finding is also pushing forward from synthetic polymers to biological macromolecules such as DNAs, RNAs, and peptides. Next, I will prepare to present our findings at upcoming conferences and submit more articles to relevant journals for more publications.As for the established timeline and budget, the research project is also proceeding according to the plan, with no significant issues or delays. I remain committed to ensuring the project's success and achieving its intended outcomes.

目前正在进行的有关固体液体界面上水溶性聚合物新盐分现象的研究项目，并有望实现既定目标。 在这里，我发现具有相同分子量的钉子，但是当其混合物的水溶液具有很高的盐与佩格重量比（〜10^6）是在玻璃板上滴铸，可以立即分离出明显的末端。 可以迅速分离四个聚合物的混合物。 即使它们的末端在结构上相似，只有两个碳原子和氢原子的差异，它们也可以迅速分离。 我已经完成了项目的重要阶段，包括文献综述，研究设计和数据收集。 收集到的数据已通过适当的科学手段进行了分析，并解释了发现。目前，我们正在努力将研究手稿重新提交给高影响力期刊，自然，其中包括对发现，结论和建议的建议，以供未来研究。 扩展这一发现的工作也将从合成聚合物转向生物大分子，例如DNA，RNA和肽。 接下来，我将准备在即将举行的会议上介绍我们的发现，并向相关期刊提交更多文章以获取更多出版物。除了既定的时间表和预算，研究项目也根据计划进行，没有重大问题或延误。我仍然致力于确保该项目的成功并取得预期的成果。