Interaction of iron species and colloids with dissolved organic matter (DOM), dissolved organic sulfur compounds (DOSC) and low-molecular weight acids (LMWA)

铁物质和胶体与溶解有机物 (DOM)、溶解有机硫化合物 (DOSC) 和低分子量酸 (LMWA) 的相互作用

• 批准号: 5454019
• 负责人: Professor Dr. Christian Zwiener
• 金额: --
• 依托单位: Arbeitsgruppe Umweltanalytik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5454019?language=en
• 关键词: Interaction; iron; species; colloids; dissolved

Specific biochemical and co-metabolic pathways of microbial degradation often form unique extracellular intermediates or metabolites, which can be used to indicate in situ electron transfer processes in aquifers. Arylsuccinate derivatives have emerged as potentially universal metabolic biomarkers for the transformation of toluene, xylene, ethylbenzene, and methylnaphthalene under anaerobic conditions. They were identified in laboratory cultures and partly in field samples. Their occurrence and distribution in the field has been neither investigated yet systematically, nor were the geochemical and hydraulic parameters investigated that may influence formation and further turnover of metabolites. Therefore, we will use in this project succinic acid derivatives and other acidic aromatic or aliphatic metabolites to monitor and understand the transformation of organic compounds by electron transfer processes. Data of the occurrence and spatial distribution of metabolites will be further used to elucidate their role as electron donors and to estimate the relevance of the electron donor function. For that purpose we will use column experiments with pure cultures to investigate the formation and turnover of specific metabolites (e.g. arylsuccinates, arylcarboxylic acids, low-molecular weight acids) and influencing parameters. The data obtained from those experiments will be used for reactive transport modeling. The calibrated model will then be used to simulate metabolite occurrence and distribution in different scenarios. Bioreactor experiments will address the question on contaminant degradation and metabolite formation/turnover with an inoculum of non-contaminated peat land under different geochemical conditions (boundary conditions). Metabolite accumulation will be of particular interest in this context. Field measurements will be performed in geochemically reactive zones of the site, where we will investigate metabolite pattern, their spatial distribution and accumulation. Analytical methods mainly with liquid chromatography-tandem mass spectrometry (LC-MS-MS) combined with different derivatization reactions will be developed to guarantee successful metabolite analysis in small sample volumes of column experiments and in groundwater samples of field sites.

微生物降解的特定生化和合作代谢途径通常形成独特的细胞外中间体或代谢产物，可用于表明含水层中的原位电子转移过程。在厌氧条件下，芳基核酸衍生物已成为甲苯，二甲苯，乙烯和甲基苯二甲烯的潜在通用代谢生物标志物。它们在实验室培养物中被鉴定出来，部分在现场样本中。它们在该领域的发生和分布尚未进行系统的研究，也没有研究地球化学和液压参数，这些参数可能影响代谢物的形成和进一步的转换。因此，我们将在该项目中使用琥珀酸衍生物和其他酸性芳香族或脂肪族代谢产物来监测和理解通过电子转移过程对有机化合物的转化。代谢物的发生和空间分布的数据将进一步用于阐明其作为电子供体的作用，并估计电子供体功能的相关性。为此，我们将使用纯培养物的柱实验来研究特定代谢产物（例如芳基核酸，芳基羧酸，低分子量酸）和影响参数的形成和周转。从这些实验获得的数据将用于反应性传输建模。然后，校准模型将用于模拟不同情况下的代谢产物出现和分布。生物反应器实验将解决有关污染物降解和代谢产物形成/更新的问题，并在不同的地球化学条件下（边界条件）接种了非污染泥炭土地的接种物。在这种情况下，代谢物积累将特别感兴趣。现场测量将在该地点的地球化学区域进行，我们将研究代谢产物模式，它们的空间分布和积累。将开发主要使用液相色谱串联质谱法（LC-MS-MS）结合不同衍生反应的分析方法，以确保在柱实验的小样本体积和田间位点的地下水样品中成功的代谢物分析。