Generation of clock oscillators in bovine endometrial cells and their functions

牛子宫内膜细胞时钟振荡器的产生及其功能

• 批准号: 22380152
• 负责人: HATTORI Masa-aki
• 金额: $ 12.31万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380152/
• 关键词: 子宮内膜細胞; 細胞時計; 代謝・内分泌制御; 時計機構; ウシ子宮内膜細胞; 出力系遺伝子; Rev-erbα; Ptgs2; プロスタグランジンF2α; ステロイドホルモン; 発情回帰; 繁殖; 遺伝子発現; 生物時計; 子宮

This study was performed to evaluate the roles of circadian clockwork in bovine uterus stromal andepithelial cells during the stages of estrous cycle and pregnancy, compared to the rat uterus cells.To analyze clock-controlled genes, we used siRNA against a core clock gene Bmal1 and an agonistor an antagonist to Rev-erbα. In the Bmal1-knockdowned stromal cells, Rev-erbα and Ptgs2expression were decreased. However, the Ptgs2 gene was increased with Rev-erbα antagonistSR8278, but not its agonist heme, in both cell types. These results strongly suggest that thesynthesis of prostaglandin is controlled under circadian clockwork

本研究的目的是与大鼠子宫细胞相比，评估动情周期和妊娠阶段牛子宫基质和上皮细胞昼夜节律的作用。为了分析时钟控制基因，我们使用针对核心时钟基因 Bmal1 的 siRNA Rev-erbα 的激动剂和拮抗剂在 Bmal1 敲低的基质细胞中，Rev-erbα 和 Ptgs2 表达降低。在两种细胞类型中，Rev-erbα 拮抗剂 SR8278 都会增加 Ptgs2 基因，但其激动剂血红素的增加程度不明显。这些结果表明前列腺素的合成受到昼夜节律的控制。

项目成果

Circadian rhythms of clockgenes and clock-controlled genes in theendometrial stroma cells prepared from thepregnant rat uterus

妊娠大鼠子宫内膜基质细胞中时钟基因和时钟控制基因的昼夜节律

• 发表时间: 2012
• 作者: ISAYAMA K; CHEN H; YAMAUCHI N;HATTORI M
• 通讯作者: HATTORI M

Pivotal role ofcircadian clock gene Bmal1 in rat luteinizinggranulosa cells

生物钟基因Bmal1在大鼠黄素化颗粒细胞中的关键作用

• 发表时间: 2012
• 作者: CHEN H; HATTORI M
• 通讯作者: HATTORI M

ウシ子宮内膜間質細胞および上皮細胞におけるペースメーカーの振動

牛子宫内膜基质和上皮细胞的起搏器振荡

• 发表时间: 2011
• 作者: 諫山慧士朗・田崎広大・山内伸彦・服部眞彰

Contribution of FSH and triiodothyronine to the development of circadian clocks during granulosa cell maturation

FSH 和三碘甲状腺原氨酸对颗粒细胞成熟过程中生物钟发育的贡献

• DOI: 10.1152/ajpendo.00470.2011
• 发表时间: 2012
• 影响因子: 5.1
• 作者: Chu G; Misawa I; Chen H; Yamauchi N; Shigeyoshi Y; Hashimoto S; Hattori M
• 通讯作者: Hattori M

Down-regulation of circadian clock geneperiod 2 in uterine endometrial stromal cells ofpregnant rats during desidualzation

妊娠大鼠子宫内膜基质细胞生物钟基因period 2的下调

• 发表时间: 2011
• 影响因子: 2.8
• 作者: UCHIKAWA M; KAWAMURA M;YAMAUCHI N; HATTORI M
• 通讯作者: HATTORI M