Study of Rapid Detection Technology for Oil Quality and Toxicological Evaluation of Frying Oil

煎炸油油质快速检测技术及毒理学评价研究

• 批准号: 17F17751
• 负责人: 惠 淑萍
• 金额: $ 1.41万
• 依托单位: Hokkaido University (2018-2019)Akita Prefectural University (2017)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2017
• 资助国家: 日本
• 起止时间: 2017-11-10 至 2020-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17F17751/
• 关键词: Hydroperoxide,; cell toxicity,; fatty acid; triacylglycerol; TGOOH; hydroperoxides; Frying oil; quality evaluation; e-nose; polar compounds; toxicity; in vitro

It was established that an effective and rapid detection method based on the odor fingerprint of frying oil for a real-time detection and monitoring of frying oil quality. The potential toxicities of frying oils' polar substances and oxidative products (hydroperoxides) to cells in vivo were investigated. The major primary oxidative products of the linoleic acid, 9 and 13-Hydroxyoctadecadienoic acid (9 and 13-HPODE), were synthesized using soybean lipoxygenase I-B. The 9 and 13-HPODE were also subjected to HepG 2 cells to evaluate its possible adverse effects (cell viability, changes in the enzyme expression, lipidomic analysis) . The research results of 2019 are summarized as follows:1.The synthesized hydroperoxides have been identified by the Mass spectroscopy and NMR. Results showed that the purity of the synthesized 13-HPODE was around 95% and the yield rate was 70% above which implies the method is successful and was feasible for preparing 9 and 13-HPODE in a regular laboratory.2.In the cells’ experiments, there were two parallel experiments carried out: one is for the PAHs and another is for the HPODEs using target cell line- HepG 2 cells. Results showed that the mRNA expression of GSTO1, SOD2, and HO-1 have increased when the cells were treated with high HPODEs concentration (100 uM), while the GPX mRNA expression was inhibited after the treatment. Results also indicated that the expression level of CYP1A1 mRNA has been significantly increased after the cells treated with high concentration of HPODEs.

建立了一种基于煎炸油气味指纹的有效快速检测方法，实时检测和监测煎炸油中极性物质和氧化产物（氢过氧化物）对体内细胞的潜在毒性。研究了亚油酸的主要初级氧化产物9和13-羟基十八二烯酸（9和13-HPODE）。大豆脂氧合酶I-B。还对HepG 2细胞进行了评估其可能的不良影响（细胞活力、酶表达的变化、脂质组学分析），2019年的研究结果总结如下：合成的氢过氧化物经质谱和核磁共振鉴定，结果表明合成的13-HPODE纯度在95%左右，收率达到95%左右。 70%以上，表明该方法是成功的，在常规实验室制备9和13-HPODE是可行的。2.细胞实验中，进行了两个平行实验：一个是针对PAHs，另一个是针对PAHs。使用靶细胞系 HepG 2 细胞进行 HPODE 结果显示，当用高 HPODE 浓度（100 uM）处理细胞时，GSTO1、SOD2 和 HO-1 的 mRNA 表达增加。结果还表明，高浓度HPODEs处理细胞后，CYP1A1 mRNA的表达水平显着升高。

项目成果

Quality evaluation of rapeseed oils based on the changes of volatile flavor and unsaturated fatty acids during frying

基于煎炸过程挥发风味和不饱和脂肪酸变化的菜籽油品质评价

• 发表时间: 2018
• 作者: Jin-Kui Ma;Tomomi Kono;Jie-Yu Chen;Han Zhang
• 通讯作者: Han Zhang

Fried oil-induced changes in xenobiotic metabolizing enzymes in human HepG2 cells

煎炸油诱导人 HepG2 细胞异生物质代谢酶的变化

• 发表时间: 2019
• 作者: Jin-Kui Ma，Wageh Sobhy Darwish;Min-Cheng Gao;Hui Tan;Hitoshi Chiba;Shu-Ping Hui
• 通讯作者: Shu-Ping Hui