Information transmission of local circuits in the primary visual cortex revealed by juxta-cellular single-cell stimulation

近细胞单细胞刺激揭示初级视觉皮层局部回路的信息传递

• 批准号: 21700433
• 负责人: TANAKA Hiroki
• 金额: $ 1.41万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700433/
• 关键词: 大脳皮質; 局所回路; 神経結合; 傍細胞電気刺激; 第一次視覚野; 視覚

Detailed description of local neural circuits in the cerebral cortex is important for understanding how visual cortices work. Here, we established a method to stimulate single cortical neuron and analyze how inputs from the neuron affect spiking activities of nearby neurons. Using juxtacellular recording and current stimulation with single glass electrodes we evoked and recorded spikes from single cells (juxta-cells) in the cat visual cortex (area 17). Simultaneously we recorded activities of neighboring cells (nearby-cells) using silicon multi-site electrodes. Current (0.5～3nA) was repetitively applied (0.5 sec ON and 0.5 sec OFF) while blank screen or sinusoidal gratings of various orientations were presented to the eyes. Single cells in the cortex alone can increase or decrease firing rate of nearby cells. The efficacy rate mostly distributes in the range of +/-0.2. For excitatory connection, the efficacy increases linearly (response map) with tuning similarities of pre-neurons (juxta-cells) and post neurons (nearby cells), whereas the efficacy for inhibitory connection does not depend on the tuning similarities.

大脑皮层局部神经回路的详细描述对于理解视觉皮层的工作原理非常重要，在这里，我们建立了一种刺激单个皮层神经元的方法，并使用细胞旁记录和电流刺激来分析神经元的输入如何影响附近神经元的尖峰活动。使用单个玻璃电极，我们诱发并记录了猫视觉皮层（区域 17）中单个细胞（并列细胞）的尖峰，同时我们记录了邻近细胞的活动。使用硅多点电极重复施加电流（0.5-3nA）（0.5秒开启和0.5秒关闭），同时将空白屏幕或各种方向的正弦光栅呈现给眼睛。单独可以增加或减少附近细胞的放电率。对于兴奋性连接，功效主要分布在+/-0.2的范围内（反应）。图）具有前神经元（并列细胞）和后神经元（附近细胞）的调整相似性，而抑制连接的功效并不依赖于调整相似性。

项目成果

nputs from single neurons in the visual cortex affect spike-timing of locally connected neurons but do not modulate their firing rate and sensory tuning curves

来自视觉皮层中单个神经元的输入影响局部连接神经元的尖峰时序，但不调节它们的放电速率和感觉调谐曲线

• 发表时间: 2010
• 作者: Tanaka H. Asada Y; Mizoguchi R; Ohzawa H; Fujita I;Tamura H.
• 通讯作者: Tamura H.

Inputs from single neurons in the visual cortex affect spike-timing of locally connected neurons but do not modulate their firing rate and sensory tuning curves

来自视觉皮层中单个神经元的输入会影响局部连接神经元的尖峰时序，但不会调节它们的放电速率和感觉调谐曲线

• 发表时间: 2010
• 作者: Tanaka; et al
• 通讯作者: et al

コントラスト空間分布を効率よく伝える機構としてのV1野高次受容野構造

V1 区域的高阶感受野结构作为有效传达对比度空间分布的机制

• 发表时间: 2010
• 作者: 田中宏喜

Inputs from single neurons in the visual cortex affect spike timing of locally connected neurons that do not modulate their firing rate and sensory tuning curve

来自视觉皮层中单个神经元的输入会影响局部连接神经元的尖峰时间，这些神经元不会调节其放电速率和感觉调谐曲线

• 发表时间: 2010
• 作者: Tanaka; H.; Asada Y.; Mizoguchi; R.; Ohzawa I.; Fujita; I.;Tamura; H.
• 通讯作者: H.

Inputs from single neurons in the visual cortex affect spike timing of locally connected neurons but do not modulate their firing rate and sensory tuning curve

来自视觉皮层中单个神经元的输入会影响局部连接神经元的尖峰时序，但不会调节它们的放电速率和感觉调谐曲线

• 发表时间: 2010
• 作者: Tanaka; et al
• 通讯作者: et al