ニホンザルとオランウータンにおける内分泌動態に影響を与える要因について

影响日本猕猴和猩猩内分泌动态的因素

• 批准号: 16J00399
• 负责人: CICALISE TAKESHI RAFAELA
• 金额: $ 1.47万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2016
• 资助国家: 日本
• 起止时间: 2016-04-22 至 2018-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16J00399/
• 关键词: Macaca fuscata; Pongo pygmaeus; DHEAS; glucocorticoids; testosterone; enzyme immunoassay; stress; endocrinology; stress hormone; Macaca fuscata; Pongo pygmaeus; DHEAS; glucocorticoids; testosterone; enzyme immunoassay; stress; endocrinology; stress hormone

I developed a new Enzyme Immunoassay (EIA) to detect dehydroepiandrosterone-sulfate (DHEAS) levels in feces of Japanese macaques and orangutans collected in the previous year. I validated the assay by confirming that DHEAS levels increased after DHEA challenge in one male and one female Japanese macaques. Additional challenges using Adrenocorticotrophic hormone (ACTH) and human chorionic gonadotropin(hGC) in other monkeys revealed that the intra-muscular injection of ACTH and hCG did not stimulate the adrenal to secrete DHEAS in Japanese macaques. The study has been published at the International Journal of Primatology. I also published the results from the previous year in the journla Primates, related to the stress-reducing effect of hot springs in stress levels of Japanese macaques.Another part of this project included the validation of the EIA for measurement of DHEAS in feces of orangutans. I investigated hormonal changes (DHEAS and glucocorticoids) levels with: (a) stress response, (b) reproductive state, and (c) age in orangutans from captivity and from the wild. The results suggest that DHEAS rises in response to increase in GC levels. I also found that pregnant females had the highest levels of DHEAS, and that early lactating females had the highest levels of fGC. Finally, I found that DHEAS is lower in infants than in adults and adolescents, with a pre-pubertal increase on DHEAS levels. This phenomenon is called adrenarche, and it is present in humans, chimpanzees, and bonobos, but not in macaques and lower primates.

我开发了一种新的酶免疫测定法（EIA），以检测上一年收集的日本猕猴和猩猩的粪便中脱氢表硫酸盐（DHEAS）水平。我通过确认在DHEA挑战后的DHEAS水平升高来验证了该测定法，其中一名男性和一名雄性日本猕猴。其他猴子中使用肾上腺皮质营养激素（ACTH）和人类绒毛膜促性腺激素（HGC）提出的其他挑战表明，ACTH和HCG的肌内注射并未刺激日本麦克拉（Macaques）刺激肾上腺。该研究已发表在《国际灵摘杂志》上。我还在《杂志灵长类动物》中发表了上一年的结果，这与温泉在日本猕猴的压力水平上的压力减轻效应有关。该项目的其他部分包括验证EIA在orangutans粪便中测量DHEA的EIA。我研究了荷尔蒙变化（DHEAS和糖皮质激素）水平：（a）应力反应，（b）生殖状态，以及（c）（c）（c）来自囚禁和野生的猩猩年龄。结果表明，DHEAS响应GC水平的增加而增加。我还发现怀孕的女性具有最高水平的DHEAS，而早期的哺乳期雌性具有最高水平的FGC。最后，我发现婴儿中的DHEAS比成年人和青少年低，而dheas水平的木星前升高。这种现象称为肾上腺素，它存在于人类，黑猩猩和bo骨中，但不在猕猴和下灵长类动物中。