Dissimilatory nitrate reduction in the coastal area

沿海地区硝酸盐异化还原

• 批准号: 20710011
• 负责人: SENGA Yukiko
• 金额: $ 2.66万
• 依托单位: Rissho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20710011/
• 关键词: 物質循環; 異化型硝酸還元; DNRA; 脱窒; 沿岸域; 亜酸化窒素(N_2O)

Kushiro Mire is the largest mire in Japan and in 1980 was the first wetland in Japan registered under the Ramsar Convention. Recent reports indicate an increase in nutrient loading into Kushiro Mire from changes in land use. We measured vertical profiles of dissolved inorganic nitrogen (DIN ; NO_3^-, NO_2^-, NH_4^+), dissolved organic carbon (DOC), and various types of microbial activity in soil samples collected to approximately 1.5m depth at two sites in Kushiro Mire. We found an accumulation of NO_3^- and DOC in the deeper soil. Denitrifying activity was highest in the shallower soils, and decreased drastically with depth, whereas higher levels of fluoresceindiacetate hydrolysis and β-glucosidase, acid phosphatase, and xylosidase enzyme activity were found in the deeper layers. We also detected humic-like substances as components of the DOC. These results suggest that the DOC in the wetland soil cannot be used as a substrate for denitrification, causing denitrification to be suppressed in the deeper soil. In addition, denitrifying activity would be very low in the deeper layers due to low soil temperature. As a result, nitrogen inputs to the Mire have resulted in a large accumulation of NO_3^- in the deeper soil. This will eventually change the mire ecosystem through effects such as increased eutrophication and acidification.

钏路泥沼是日本最大的泥沼，1980 年是日本第一个根据拉姆萨尔公约注册的湿地。最近的报告表明，由于土地利用的变化，钏路泥沼的养分负荷有所增加。 ；NO_3^-、NO_2^-、NH_4^+)、溶解有机碳 (DOC) 以及采集至约 1.5m 深度的土壤样品中的各类微生物活性在钏路泥沼的两个地点，我们发现较浅的土壤中NO_3^-和DOC的积累最高，并且随着深度的增加而急剧下降，而荧光素二乙酸酯水解和β-葡萄糖苷酶、酸性磷酸酶的水平较高。我们还在深层中发现了腐殖质物质和木糖苷酶活性，这些结果表明 DOC 中存在。湿地土壤不能作为反硝化的基质，导致深层土壤的反硝化受到抑制，而且由于土壤温度较低，深层的反硝化活性很低，导致沼泽的氮输入量减少。导致深层土壤中NO_3^-的大量积累，这最终将通过增加富营养化和酸化等影响来改变泥沼生态系统。

项目成果

Vertical profiles of DIN, DOC and microbial activities in the peat soilin Kushiro Mire, northeastern Japan

日本东北部钏路泥炭土中 DIN、DOC 和微生物活动的垂直剖面

• 发表时间: 2011
• 影响因子: 1.6
• 作者: Senga Y; Hiroki M; Nakamura Y; Watarai Y; Watanabe Y; Nohara S
• 通讯作者: Nohara S

砂泥堆積物中溶存硫化物の簡便な現場抽出/吸光光度定量及びその有明海北東部堆積物への適用

简单现场萃取/分光光度法测定沙泥沉积物中溶解的硫化物及其在有明海东北部沉积物中的应用

• 发表时间: 2010
• 作者: 管原庄吾; 圦本達也; 鮎川和泰; 木元克則; 千賀有希子; 奥村稔; 清家泰
• 通讯作者: 清家泰

Seasonal and spatial variation in the denitrifying activity in estuarine and lagoonal sediments.

河口和泻湖沉积物反硝化活动的季节和空间变化。

• 发表时间: 2010
• 作者: Senga Y; Okumura M; Seike Y
• 通讯作者: Seike Y

汚濁池沼への酸素供給が有機物分解および微生物群集へ与える影響

污染池塘供氧对有机物分解和微生物群落的影响

• 发表时间: 2010
• 作者: 千賀有希子; 渡辺泰徳
• 通讯作者: 渡辺泰徳

汚濁池沼への空気供給が窒素および炭素動態に与える影響

污染池塘供气对氮和碳动态的影响

• 发表时间: 2009
• 作者: 千賀有希子; 渡辺泰徳
• 通讯作者: 渡辺泰徳