Structural analysis of cytoskeletal regulatory protein

细胞骨架调节蛋白的结构分析

• 批准号: 20770093
• 负责人: SUETSUGU Kyoko
• 金额: $ 2.75万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20770093/
• 关键词: 細胞骨格; X線構造解析; 細胞膜; タンパク質; シグナル伝達; 低分子Gタンパク質; アクチン; X線結晶構造解析; 細胞運動

We solved the crystal structures of N-WASP binding protein ; EFCLpacsin2 (residues 1-343 ; EFC/F-BAR domain + C-terminal extension) and EFCSpacsin2 (residues1-305 ; EFC/F-BAR domain) at 2.7 and 2.0 A resolutions, respectively. We found that overexpression of the pacsin2 EFC/F-BAR domain induced cellular microspikes, with the pacsin2 EFC/F-BAR domain concentrated at the neck. The hydrophobic loops and the basic amino-acid residues on the concave surface of the pacsin2 EFC/F-BAR domain are essential for both the microspike formation and tubulation. Since the curvature of the neck of the microspike and that of the tubulation share similar geometry, the pacsin2 EFC/FBAR domain is considered to facilitate both microspike formation and tubulation.

我们解析了N-WASP结合蛋白的晶体结构； EFCLpacsin2（残基 1-343；EFC/F-BAR 结构域 + C 端延伸）和 EFCSpacsin2（残基 1-305；EFC/F-BAR 结构域）分辨率分别为 2.7 和 2.0 A。我们发现 pacsin2 EFC/F-BAR 结构域的过度表达会诱导细胞微尖峰，其中 pacsin2 EFC/F-BAR 结构域集中在颈部。 pacsin2 EFC/F-BAR 结构域凹表面上的疏水环和碱性氨基酸残基对于微刺形成和管状化至关重要。由于微刺颈部的曲率和管状结构具有相似的几何形状，因此 pacsin2 EFC/FBAR 域被认为有利于微刺形成和管状化。

项目成果

Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II.

pacsin2/Syndapin II 的 EFC/F-BAR 结构域负责管管和细胞突出的基本氨基酸残基的定位。

• 发表时间: 2010
• 作者: Shimada A; Takano K; Shirouzu M; Hanawa
• 通讯作者: Hanawa

Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II

pacsin2/Syndapin II 的 EFC/F-BAR 结构域负责管管和细胞突出的基本氨基酸残基的定位

• 作者: Atsushi Shimada; et al.
• 通讯作者: et al.

Structural basis for the exclusive specificity of Slac2-a/melanophilin for the Rab27 GTPases.

Rab27 GTPases 的 Slac2-a/促黑素的唯一特异性的结构基础。

• 发表时间: 2008
• 作者: Kukimoto