Neurobehavioral changes in metallothionein-null mice postnatal exposed to low levels mercury vapor.

出生后暴露于低水平汞蒸气的金属硫蛋白缺失小鼠的神经行为变化。

• 批准号: 20590611
• 负责人: YOSHIDA Minoru
• 金额: $ 3.08万
• 依托单位: Hachinohe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20590611/
• 关键词: 神経行動毒性; 水銀蒸気曝露; 新生児; メタロチオネイン; マイクロアレイ; 無機水銀; 水銀蒸気; マウス; 脳内水銀濃度; 加齢

Metallothionein MT-null and wild-type mice were repeatedly exposed to Hg^0 at 0.057 mg/m^3, which were similar to the current threshold value (TLV), until the 20th of postpartum. Hg^0-exposed mice of both strains did not show behavioral changes such as total locomotor activity, learning ability and spatial learning ability. However, Hg^0 exposure resulted in significant alterations in gene expression in the brain of both strains using DNA microarray analysis. A number of altered genes in MT-null mice were more than those in wild-type mice. When the exposure levels elevated to 0.198mg/m^3, MT-null mice showed a significant decrease in total locomotor activity in female, though learning ability and spatial learning ability were not affected. A number of altered genes were further increased by elevation of Hg^0 exposure levels. DNA microarray analysis may provide useful information to elucidate development of behavioral toxicity following a low-level exposure to Hg^0.

金属硫蛋白MT缺失和野生型小鼠重复暴露于0.057 mg/m^3的Hg^0，其与当前阈值（TLV）相似，直到产后20日。两种品系的 Hg^0 暴露小鼠均未表现出行为变化，如总运动活动、学习能力和空间学习能力。然而，通过 DNA 微阵列分析，Hg^0 暴露导致两种菌株大脑中基因表达的显着改变。 MT缺失小鼠中的一些改变基因数量比野生型小鼠更多。当暴露水平升高至0.198mg/m^3时，雌性MT缺失小鼠的总运动活动显着下降，但学习能力和空间学习能力未受影响。 Hg^0 暴露水平的升高进一步增加了许多改变的基因。 DNA 微阵列分析可以提供有用的信息来阐明低水平接触 Hg^0 后行为毒性的发展。

项目成果

Neurobehavioral effects of combined prenatal exposure to low-level mercury vapor and methylmercury.

产前联合暴露于低水平汞蒸气和甲基汞对神经行为的影响。

• 发表时间: 2011
• 作者: Yoshida M; Suzuki M; Satoh M; Yasutake A; Watanabe C.
• 通讯作者: Watanabe C.

Emergence of delayed methylmercury toxicity after perinatal exposure in metallothionein-null and wild-type C57BL mice.

金属硫蛋白无效和野生型 C57BL 小鼠围产期暴露后出现迟发性甲基汞毒性。

• 发表时间: 2008
• 作者: Yoshida M; Shimizu N; Suzuki M; Watanabe C; Satoh M; Mori K; Yasutake A.
• 通讯作者: Yasutake A.

新生仔MTノックアウトマウスの水銀蒸気に対する感受性について

新生MT敲除小鼠对汞蒸气的敏感性

• 发表时间: 2010
• 作者: 吉田稔

マウスにおける無機水銀の神経毒性作用:無機水銀脳室内投与モデルを用いた検討

无机汞对小鼠的神经毒性作用：使用无机汞脑室内给药模型的研究

• 发表时间: 2009
• 作者: 吉田稔;安武章
• 通讯作者: 安武章

Neurobehavioral effects of combined prenatal exposure to low-level mercury vapor and methylmercury.

产前联合暴露于低水平汞蒸气和甲基汞对神经行为的影响。

• 发表时间: 2011
• 作者: Yoshida M; Suzuki M; Satoh M; Yasutake A; Watanabe C.
• 通讯作者: Watanabe C.