Identification of neurons by fluorescence spectrogram through a patch electrode, and the application to auditory neural circuits in vivo

贴片电极荧光光谱识别神经元及其在体内听觉神经回路中的应用

• 批准号: 20220008
• 负责人: OHMORI Harunori
• 金额: $ 105.75万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20220008/
• 关键词: パッチ電極; 神経細胞; 蛍光蛋白; 神経回路; 聴覚; 蛍光トレーサー; 蛍光電極; パッチクランプ; 分光器; 聴覚神経回路; オレゴングリーンBAPTA; Field-L; FRET; 蛍光分光; 蛍光タンパク

This study aimed at recording optical signal simultaneously with neuronal electrical activity in the deep brain tissue. For that purpose, we developed a photometric patch electrode (PME) recording system by using a patch electrode as a light guide. By filling the neuron in the auditory nuclei of a chick with Oregon green BAPTA-1-AM (OGB-1), intracellular Ca2+signals were recorded simultaneously with electrical signal in response to acoustic stimulus in the Field-L, the avian auditory cortex, and in the inferior colliculus. A slow field current was enhanced after the block of GABA receptor in the Field-L, which enhanced the Ca2+wave that was generated in synchrony with the electrical slow wave. In the inferior colliculus, Ca2+responses were similarly recorded and were enhanced after the block of GABA receptor. Therefore, we succeeded in development of PME recording system to monitor neuronal electrical activity simultaneously with optical signal through the patch electrode in the deep brain tissue.

这项研究旨在同时记录深部脑组织中的光信号和神经元电活动。为此，我们开发了一种光度贴片电极（PME）记录系统，使用贴片电极作为光导。通过用俄勒冈绿 BAPTA-1-AM (OGB-1) 填充小鸡听觉核中的神经元，细胞内 Ca2+ 信号与电信号同时记录，以响应 Field-L（鸟类听觉皮层）中的声刺激，以及下丘。阻断Field-L中的GABA受体后，慢场电流增强，从而增强了与电慢波同步产生的Ca2+波。在下丘中，Ca2+反应也有类似的记录，并且在GABA受体阻断后增强。因此，我们成功开发了PME记录系统，通过脑组织深处的贴片电极与光信号同时监测神经元电活动。

项目成果

Activation of metabotropic glutamate receptors improves the accuracy of coincidence detection by presynaptic mechanisms in the nucleus laminaris of the chick.

代谢型谷氨酸受体的激活提高了雏鸡层状核中突触前机制的重合检测的准确性。

• DOI: 10.1113/jphysiol.2012.244350
• 发表时间: 2013
• 作者: Okuda H; Yamada R; Kuba H & Ohmori H
• 通讯作者: Kuba H & Ohmori H

時間差の検出と計算機シミュレーション

时差检测与计算机模拟

• 发表时间: 2013
• 作者: 福井 巌

Sound intensity dependent compensation for the small ITD cue in the chicken.

对鸡中的小 ITD 提示进行声音强度相关补偿。

• 发表时间: 2009
• 作者: Ohmori H.

Interaural phase difference modulates the neural activity in the nucleus angularis and improves the processing of level difference cue in the lateral lemniscal nucleus in the chicken.

耳间相位差调节角核的神经活动，并改善鸡外侧丘系核的水平差线索的处理。

• 发表时间: 2010
• 作者: Sato T; Fukui I; Ohmori H.
• 通讯作者: Ohmori H.

Presynpatic activity regulates Na+ channel distribution at the axon intial segment

突触前活动调节轴突初始段的 Na 通道分布

• 发表时间: 2010
• 影响因子: 64.8
• 作者: Kuba H; Oichi Y; Ohmori H
• 通讯作者: Ohmori H