Monitoring of Inflammatory Markers in Exhaled Breath Condensate in patients with Asthma and Development of Evaluating System of Asthma Severity

哮喘患者呼出气冷凝液中炎症标志物的监测及哮喘严重程度评估系统的开发

基本信息

批准号：
19590560
负责人：
KATAOKA Mikio
金额：
$ 2.83万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2007
资助国家：
日本
起止时间：
2007 至 2009
项目状态：
已结题

项目摘要

The evaluation of airway inflammation is important for the diagnosis and treatment of asthma. The exhaled breath condensate (EBC) is a less invasive method compared with bronchoalveolar lavage or bronchial biopsy. Measurement of mediators in EBC could be a useful method for monitoring airway inflammation in asthma. The aims of this study are to establish the EBC collection method and to find measurable biomarkers reflecting asthmatic airway inflammation and the relationship between these biomarkers and asthmatic severity and lung function. Fifty-eight non-smoking healthy subjects, 7 asymptomatic smokers, 9 common cold subjects and 55 asthmatics with disease severity ranging from mild intermittent to severe persistent were enrolled. We measured the levels of pH, CRP, albumin, Eosinophil cationic protein (ECP), H_2O_2, and nitrite/nitrate in EBC. The levels of CRP, H_2O_2, nitrite/nitrate of the asthma group were significantly higher than in the control group. In asthmatic severity, the levels of pH, CRP, ECP, H_2O_2 and nitrate in the mild persistent group were significantly higher than the other groups with severity. In addition, the H_2O_2 level in EBC significantly correlated with that of nitrite/nitrate. FEV1 and PEFR showed a significant negative correlation with the levels of ECO, H_2O_2 and nitrite/nitrate. EBC collection is noninvasive and useful to evaluate airway inflammation by measuring inflammatory markers in the management of asthma.

气道炎症的评估对于哮喘的诊断和治疗具有重要意义。与支气管肺泡灌洗或支气管活检相比，呼出气冷凝液（EBC）是一种侵入性较小的方法。 EBC 中介质的测量可能是监测哮喘气道炎症的有用方法。本研究的目的是建立EBC采集方法并寻找反映哮喘气道炎症的可测量生物标志物以及这些生物标志物与哮喘严重程度和肺功能之间的关系。纳入 58 名不吸烟健康受试者、7 名无症状吸烟者、9 名普通感冒受试者和 55 名病情严重程度从轻度间歇性到重度持续性的哮喘患者。我们测量了 EBC 中的 pH、CRP、白蛋白、嗜酸性粒细胞阳离子蛋白 (ECP)、H_2O_2 和亚硝酸盐/硝酸盐的水平。哮喘组CRP、H_2O_2、亚硝酸盐/硝酸盐水平显着高于对照组。在哮喘严重程度方面,轻度持续组的pH、CRP、ECP、H_2O_2和硝酸盐水平显着高于其他严重组。此外，EBC中H_2O_2水平与亚硝酸盐/硝酸盐水平显着相关。 FEV1和PEFR与ECO、H_2O_2和亚硝酸盐/硝酸盐水平呈显着负相关。 EBC 采集是非侵入性的，可通过测量哮喘治疗中的炎症标志物来评估气道炎症。