Bioethanol production from bamboo culms using selective lignin-degrading basidiomycetes

利用选择性木质素降解担子菌从竹秆生产生物乙醇

• 批准号: 24710085
• 负责人: SUHARA Hiroto
• 金额: $ 3万
• 依托单位: Miyazaki Prefectural Wood Utilization Research Center
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24710085/
• 关键词: バイオエタノール; 担子菌類; タケ; イネ科植物; 選択的脱リグニン; 選択的リグニン分解

Pretreatment for bioethanol production from bamboo culms and rice straw was examined using basidiomycetous fungus Punctularia sp. TUFC20056, which has selective lignin removal ability of bamboo. A class II peroxidase gene was partially obtained from this fungus, and it codes manganese peroxidase (MnP). Optimal cultural conditions for producing lignin-degrading enzymes were as follows; laccase was 21- 25 C, 3-4 weeks or more, on solid culture condition; MnP was 21- 25 C, 3 weeks, in liquid culture.Fermentable sugar yields from Punctularia sp.-treated bamboo (10%) and rice straw (46%) were higher than that of famous lignin degrader Phanerochaete sordida (2.4 and 8.9%, respectively) and Ceriporiopsis subvermispora. (6.1 and 9.7%, respectively). Punctularia sp. cannot ferment cellulose directly. But, this fungus can produce ethanol by adding cellulase under an anerobic condition, and the yields from pretreated-bamboo and -rice straw were 25.2 and 37.5%, respectively.

使用culm茎和稻草生产生物乙醇的预处理，并使用粘液菌真菌sp。 TUFC20056，具有竹子的选择性木质素去除能力。从该真菌中部分获得了II类过氧化物酶基因，并代码锰过氧化物酶（MNP）。产生木质素降解酶的最佳文化条件如下；在固体培养条件下，漆酶为21-25 C，3-4周或更长时间。在液体培养中，MNP为21-25 c，3周。细胞状态治疗的竹子（10％）和稻草（46％）的糖产量高于著名的木质素降解蛋白降解蛋白Phanerochaete sordida（分别为2.4和8.9％）和ceriporiporioopsis subvermispora。 （分别为6.1和9.7％）。点状sp。不能直接发酵纤维素。但是，这种真菌可以通过在厌氧条件下添加纤维素酶来产生乙醇，而预处理的bamboo和-rice稻草的产量分别为25.2和37.5％。

项目成果

竹リグニン選択的分解菌を用いた脱リグニン-同時糖化発酵

竹木质素选择性降解菌脱木质素同步糖化发酵

• 发表时间: 2012
• 作者: 上原星太;園部美佳;須原弘登;亀井一郎;目黒貞利
• 通讯作者: 目黒貞利

Screening of selective lignin-degrading basidiomycetes and biological pretreatment for enzymatic hydrolysis of bamboo culms

• DOI: 10.1016/j.ibiod.2012.05.042
• 发表时间: 2012-11-01
• 期刊: INTERNATIONAL BIODETERIORATION & BIODEGRADATION
• 影响因子: 4.8
• 作者: Suhara, Hiroto;Kodama, Sayaka;Meguro, Sadatoshi
• 通讯作者: Meguro, Sadatoshi

新規リグニン分解菌とその利用

新型木质素降解菌及其用途

• 发表时间: 2010