The Role of Sleep in the Reorganization of the Cerebral Cortex

睡眠在大脑皮层重组中的作用

• 批准号: 23700464
• 负责人: KARASHIMA Akihiro
• 金额: $ 2.41万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700464/
• 关键词: 睡眠; ノンレム睡眠; 大脳皮質; シナプス可塑性; AMPA受容体; 神経回路; 覚醒; グルタミン酸; 受容体

Recently, the relationship between sleep-wake rhythm and synaptic plasticity is investigated by many sleep researchers. They suggest that the synaptic connection became stronger during wakefulness and became weaker during sleep. In order to confirm this suggestion, we performed whole-cell patch-clamp recordings from layer II/III pyramidal neurons of somatosensory cortex in acute slices of the rats that had been awake or asleep. We found that i) current-voltage relationship of EPSCs showed inward rectification, and ii) eEPSCs were depressed by Philanthotoxin 74, which is antagonist of Ca2+-permeable AMPA receptors, in slices of spontaneously waking and sleep-deprived animals. Both results show that amount of Ca2+permeable AMPA receptors, which plays crucial role in synaptic plasticity, are high during wakefulness. These strongly suggest that synapses become widely potentiated during wakefulness and were downscaled during sleep.

最近，许多睡眠研究人员研究了睡眠-觉醒节律与突触可塑性之间的关系。他们认为，突触连接在清醒时变得更强，而在睡眠时变得更弱。为了证实这一建议，我们对醒着或睡着的大鼠的急性切片中的体感皮层 II/III 层锥体神经元进行了全细胞膜片钳记录。我们发现，i) EPSC 的电流-电压关系表现出内向整流，ii) 在自发清醒和睡眠剥夺的动物切片中，eEPSC 被 Philanthotoxin 74（Ca2+ 渗透性 AMPA 受体的拮抗剂）抑制。这两项结果都表明，在突触可塑性中起关键作用的 Ca2+ 渗透性 AMPA 受体的数量在清醒期间很高。这些强烈表明，突触在清醒时广泛增强，而在睡眠期间减弱。

项目成果

Reconstruction of fetal vector electrocardiogram from maternal abdominal signals under fetus body rotations

胎儿身体旋转下母体腹部信号重建胎儿矢量心电图

• DOI: 10.1109/embc.2013.6611253
• 发表时间: 2013
• 期刊: Conf. Proc. IEEE Eng. Med. Biol. Soc.
• 作者: Y. Nabeshima;Y. Kimura;T. Ito;K. Ohwada;A. Karashima;N. Katayama;M. Nakao
• 通讯作者: M. Nakao

Rapid Eye Movement Sleep : Regulation and Function (Neural modeling for cooperative/competitive regulation of REM sleep with NREM sleep and wakefulness)

快速眼动睡眠：调节和功能（快速眼动睡眠与非快速眼动睡眠和觉醒的合作/竞争性调节的神经模型）

• 发表时间: 2011
• 作者: A. Karashima;Y. Tamakawa;Y. Koyama;N. Katayama
• 通讯作者: N. Katayama

体性感覚野応答の睡眠-覚醒状態依存的変化

体感皮层反应的睡眠-觉醒状态依赖性变化

• 发表时间: 2013
• 作者: Mills SL;Tian LM;Hoshi H;Whitaker CM;Massey SC;辛島彰洋ら
• 通讯作者: 辛島彰洋ら

睡眠-覚醒状態に依存した大脳皮質シナプス可塑性

皮质突触可塑性依赖于睡眠-觉醒状态

• 发表时间: 2013
• 作者: 郭 暁麗;原田知加子;行方和彦;木村敦子;三田村佳典;吉田 寛;松本 陽;原田高幸;辛島彰洋ら
• 通讯作者: 辛島彰洋ら

Towards in silico modeling of suprachiasmatic nucleus

视交叉上核的计算机模拟

• 发表时间: 2011
• 作者: M. Nakao;A. Karashima;N. Katayama
• 通讯作者: N. Katayama