Remineralization of preliminarily carious by enamel proteins with artificial body fluids

人工体液通过牙釉质蛋白对初步龋齿进行再矿化

• 批准号: 23659924
• 负责人: MIYAZAKI Takashi
• 金额: $ 2.33万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659924/
• 关键词: エナメル質; 初期齲蝕; 再石灰化; エナメル; 再生; バイオメカニクス; エナメルタンパク

Background: Management of human teeth has moved from a surgical to a more conservative approach of inhibiting or preventing lesion progression. Increasing enamel mineralization is crucial in this regard. A potential difficulty is the preferential mineralization of the outermost portion of the enamel that can prevent overall mineralization. We describe a strategy for increasing the mineralization potential of dental enamel. Extracted human premolar teeth enamel were exposed to a high concentration of hydrogen peroxide with an energizing source. Samples were stored in artificial saliva at 37uC for 1 wk. A desktop X-ray micro-CT system was used to evaluate the mineral density of samples. Mineral distribution was polarized between the lower and the higher mineralized portion of enamel by charged oxygen free radicals due to activation of permeated hydrogenperoxide. The kinetics of energy absorption in the deeper enamel region demonstrated improvement of preferential mineralization into the region without restricting overall mineralization of the enamel. Subsequent increasing mineralization, even in the dense mineralized outer portion of enamel, was also achieved. This increased mineralization may promote resistance to acidic deterioration of the structure. The present study is one of the primary steps towards the development of novel application in reparative and restorative dentistry.

背景：人类牙齿的治疗已经从手术转向更保守的抑制或预防病变进展的方法。在这方面，增加牙釉质矿化至关重要。一个潜在的困难是牙釉质最外层部分的优先矿化，这可能会阻止整体矿化。我们描述了一种增加牙釉质矿化潜力的策略。将提取的人类前磨牙牙釉质暴露于高浓度的过氧化氢和能量源中。样品在 37uC 的人工唾液中保存 1 周。使用台式 X 射线显微 CT 系统评估样品的矿物密度。由于渗透的过氧化氢的活化而导致带电氧自由基，矿物质分布在牙釉质的较低矿化部分和较高矿化部分之间极化。较深牙釉质区域的能量吸收动力学表明，该区域的优先矿化得到改善，而不会限制牙釉质的整体矿化。随后，甚至在牙釉质的致密矿化外部部分，也实现了矿化的增加。这种增加的矿化可以促进结构对酸性劣化的抵抗力。本研究是开发修复和修复牙科新应用的主要步骤之一。

项目成果

Tape-cast and sintered β-tricalcium phosphate laminates for biomedical applications: Effect of milled Al2_O_3 fiber additives on microstructural and mechanical properties

用于生物医学应用的流延和烧结 β-磷酸三钙层压板：研磨 Al2_O_3 纤维添加剂对微观结构和机械性能的影响

• 发表时间: 2012
• 期刊: Journal of Biomedical Materials Research Part B: Applied Biomaterials
• 作者: Tanimoto Y.;Teshima M.;Nishiyama N.;Yamaguchi M.;Hirayama S.;Shibata Y.;Miyazaki T.
• 通讯作者: Miyazaki T.

A new modified laser pretreatment for porcelain zirconia bonding

• DOI: 10.1016/j.dental.2013.03.002
• 发表时间: 2013-05-01
• 期刊: DENTAL MATERIALS
• 影响因子: 5
• 作者: Liu, Dan;Matinlinna, Jukka P.;Kan, Chi-Wai
• 通讯作者: Kan, Chi-Wai

Antioxidant and osteogenic properties of anodically oxidized titanium

• DOI: 10.1016/j.jmbbm.2012.01.016
• 发表时间: 2012-09-01
• 期刊: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
• 影响因子: 3.9
• 作者: Iwai-Yoshida, Misato;Shibata, Yo;Miyazaki, Takashi
• 通讯作者: Miyazaki, Takashi

ナノインデンターによるエナメル質の物理的評価

使用纳米压痕仪对牙釉质进行物理评估

• 发表时间: 2012
• 作者: 山口幹代;野杁由一郎;田中玲奈
• 通讯作者: 田中玲奈

In vitro demineralization of tooth enamel subjected to two whitening regimes

两种美白方案对牙釉质的体外脱矿作用

• 发表时间: 2013
• 期刊: The Journal of The American Dental Association
• 作者: Ogura K,Tanaka R;Shibata Y;Miyazaki T;Hisamitsu H
• 通讯作者: Hisamitsu H