MRI of the brain in a primate model of severe human malaria

严重人类疟疾灵长类动物模型的大脑 MRI

基本信息

批准号：
18590404
负责人：
KAWAI Satoru
金额：
$ 2.57万
依托单位：
Dokkyo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590404/
关键词：
MRI cerebral malaria Plasmodium coatneyi primate model severe human malaria マラリア疾患モデル

项目摘要

Our previous studies demonstrated that the Japanese macaque (Macaca fuscata) is highly susceptible to Plasmodium coatneyi infection and that pathologic findings in infected monkeys are similar to those observed in human falciparum malaria with cerebral involvement. To elucidate the clinical features of cerebral malaria and correlation with pathologic findings, we performed magnetic resonance imaging (MRI) analysis on the brain in our primate model. In the experiment, we prepared four J-macaques (J52, J53, J54 and J55), and they were examined by four times MR imaging before and after infection. After the 2nd imaging, the macaques were autopsied and processed for pathological examination. Parasitemia of J53 was 30.1% on day 14, and the macaque exhibited serious clinical signs such as restlessness and depression. However, MRI revealed no abnormalities. Histopathological examination showed preferential sequestration of PRBCs in the cerebral and cerebellum capillaries. However, parenchymal damages such as necroris and peri-vasculare edema were not found in the section. We therefore conclude that although sequestration in the microvessels was frequently seen in our primate model during acute malarial attack, the MRI abnormality of the brain is not common in our primate model during acute phase of severe malaria.

我们之前的研究表明，日本猕猴（Macaca fuscata）对科特尼疟原虫感染高度敏感，感染猴子的病理结果与脑部受累的人类恶性疟疾中观察到的病理结果相似。为了阐明脑型疟疾的临床特征以及与病理结果的相关性，我们对灵长类动物模型的大脑进行了磁共振成像（MRI）分析。实验中，我们准备了四只J猕猴（J52、J53、J54和J55），在感染前后对它们进行了四次MR成像检查。第2次成像后，对猕猴进行尸检并进行病理检查。第14天，J53的寄生虫血症为30.1%，猕猴表现出烦躁、抑郁等严重的临床症状。然而，核磁共振检查未发现任何异常。组织病理学检查显示PRBC优先隔离在大脑和小脑毛细血管中。但切片未发现坏死、血管周围水肿等实质损伤。因此，我们得出的结论是，尽管在急性疟疾发作期间我们的灵长类动物模型中经常看到微血管隔离，但在严重疟疾急性期期间我们的灵长类动物模型中大脑的 MRI 异常并不常见。