Research on the mechanism of stimulated mucin secrefion by dietary fiber ingestion and its physiological effects

膳食纤维摄入促进粘蛋白分泌的机制及其生理效应研究

• 批准号: 18580116
• 负责人: MORITA Tatsuya
• 金额: $ 2.3万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580116/
• 关键词: mucin; dietary fiber; settling volume in water; bulk-forming effect; dietary antigen; bulk-formingeffect

The aim of this study was to clarify the mechanism of stimulated mucin secretion by dietary fiber ingestion and its physiological effects. In summary, the present research program clarified the followings:1. The small intestinal and colonic mucins are secreted in proportion to the settling volume in water (a numerical representation for bulk-forming property) of dietary indigestible components.2. Stimulated mucin secretion by insoluble dietary fiber reaches its maximum at 5 days after starting IDF ingestion and disappears at 5 days after ceasing insoluble dietary fiber ingestion, suggesting that this stimulatory effect is accordance with the replenishing cycle of epithelial cells.3. The measurement of O-linked oligosaccharide chain as a mucin marker is highly correlated with mucins directly determined by a newly established ELISA.4. Insoluble dietary fiber exerts a significant increase in luminal mucin content, but the increased capacity of luminal mucin secretion itself does not disturb the nutrient absorption.

本研究的目的是阐明膳食纤维摄入刺激粘蛋白分泌的机制及其生理效应。综上所述，本研究计划明确了以下几点： 1．小肠和结肠粘蛋白的分泌与膳食中不可消化成分在水中的沉降体积（体积形成特性的数字表示）成比例。2．不溶性膳食纤维刺激的粘蛋白分泌在开始摄入IDF后5天达到最大，并在停止不溶性膳食纤维摄入后5天消失，表明这种刺激作用与上皮细胞的补充周期一致。3． O-连接寡糖链作为粘蛋白标记物的测量与新建立的ELISA直接测定的粘蛋白高度相关。4。不溶性膳食纤维显着增加管腔粘蛋白含量，但管腔粘蛋白分泌能力的增加本身并不干扰营养吸收。

项目成果

Long-term ingestion of insoluble dietary fiber increases lurminal mucin content, but has no effects on nutrient absorption in rats

长期摄入不溶性膳食纤维可增加大鼠腔内粘蛋白含量，但对大鼠营养吸收无影响

• 发表时间: 2008
• 作者: Hiroki Tanabe

Increased Luminal Mucin Does Not Disturb Glucose or Ovalbumin Absorption in Rats Fed Insoluble Dietary Fiber

增加的管腔粘蛋白不会干扰喂食不溶性膳食纤维的大鼠的葡萄糖或卵清蛋白吸收

• 发表时间: 2006
• 作者: Tatsuya Morita

Partial Replacement of Waxy Cornstarch by Recrystallized Amylose Retards the Development of Insulin Resistance in Rats

用重结晶直链淀粉部分替代蜡质玉米淀粉可延缓大鼠胰岛素抵抗的发展

• 发表时间: 2006
• 作者: Tatsuya Morita

Long-Term Ingestion of Insoluble Dietary Fiber Increases Luminal Mucin Content, but Has No Effect on Nutrient Absorption in Rats

长期摄入不溶性膳食纤维会增加大鼠管腔粘蛋白含量，但对大鼠营养吸收没有影响

• DOI: 10.1271/bbb.70643
• 发表时间: 2008-03-23
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: T. Morita;H. Tanabe;Hiroyuki Ito;K. Sugiyama;S. Kiriyama
• 通讯作者: S. Kiriyama

In Vitro and in vivo digestibility of native maize starch granules varying inamylose contents

不同直链淀粉含量的天然玉米淀粉颗粒的体外和体内消化率

• 发表时间: 2007
• 作者: Tatsuya Morita