Function of Mugineic acid in absorption and translocation of micro elements in graminaceous plants

麦根酸在禾本科植物微量元素吸收和转运中的作用

基本信息

批准号：
18580058
负责人：
KAWAI Shigenao
金额：
$ 2.49万
依托单位：
Iwate University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18580058/
关键词：
Arsenic Nickel Mugineic acid Hydroponical Culture Barley Xylem Sap Formation of Chelate Vesicle of Plasma Membrane 吸収原子吸光光度計シオチガヤ

项目摘要

1. Relationship between release of mugineic acid (MA) and toxicity of metal micronutrient (Ni) Radioactive ^<63>Ni were fed together with MA to the barley grown hydroponically and activation of Ni absorption by MA was concluded. Absorption of ^<59>Fe was repressed by coexisting Ni in the presence of MA. Furthermore, Absorption of ^<63>Ni was not repressed by coexisting Fe. The results indicated that Ni is preferentially absorbed to Fe in the form of MA complex.2. Relationship release of MA and harmful elements (Cd, As, Co) In order to confirm that the cause of As-induced chlorosis is Fe-deficiency in barley grown hydroponically, plants were grown in the medium with the elevated Fe concentration. As a result, As-induced chlorosis was alleviated. The main cause of As-induced chlorosis was concluded to be Fe-deficiency.3. Amount of translocated micronutrient in xylem tube and effect of fed MA Manganese, Zn, Cu and with excess concentrations were fed to the barley grown hydroponically with … More or without MA for 3 hours in the afternoon, and in subsequent hours the concentrations of the metals in xylems sap was measured. The results showed that in normal plants only the concentration of Fe was increased by feeding of MA, but in Mn, Zn, or Cu toxic plants MA could increase of Cu or Zn concentration of xylem sap. It was suggested that in the Cu or Zn toxic plants released MA might aggravate Cu or Zn-induced Fe-deficiency.4. Clarification of the form of MA-metals chelates By the analysis of NMR, it was concluded that molar ratio of MA and metals, such as Fe^<3+>, Cu^<2+>, Zn^<2+>, Mn^<2+>, Ni^2, when chelates were formed, was 1:1. Furthermore, the order of strength of formation was Cu^<2+>>Fe^<3+>>Ni^<2+>>Mn^<2+>>Zn^<2+>.5. Examination of absorption of trace elements in the isolated vesicles of plasma membrane In the experiment with isolated vesicles of plasma membrane prepared from roots of Fe-deficient barely, absorption of Cd was repressed by the presence of Cu or Fe. The repression effect of Cu was larger than that of Fe. It was considered that it may be attributed to characteristic of the vesicles of plasma membrane to Fe and Cu. Less

1.麦根酸(MA)的释放与金属微量营养素(Ni)的毒性之间的关系将放射性^<63>Ni与MA一起喂入水培大麦中，并得出MA对Ni吸收的激活^<59的吸收。在MA存在下，Fe被共存的Ni抑制。此外，^ 63 Ni的吸收未被共存的Fe抑制。结果表明，Ni优先以Fe的形式被吸收。 2. MA 释放与有害元素（Cd、As、Co）的关系为了证实水培大麦缺铁是 As 引起的失绿的原因，将植物培养在高浓度的培养基中。结果表明，砷引起的失绿现象得到了缓解，认为缺铁是造成砷失绿的主要原因。 3．下午，将过量浓度的锰、锌、铜喂入水培大麦中，使用或不使用MA，持续3小时，并在随后的几个小时内测量木质部汁液中金属的浓度。结果显示，在正常情况下。在植物中，通过饲喂MA仅增加了Fe的浓度，但在Mn、Zn或Cu有毒植物中，MA可以增加木质部汁液中的Cu或Zn浓度，这表明在Cu或Zn有毒植物中释放MA。 4．MA-金属螯合物形态的澄清通过NMR分析，得出MA与Fe^3+、Cu^等金属的摩尔比。形成螯合物时<2+>、Zn^<2+>、Mn^<2+>、Ni^2为1:1。而且，形成的强度顺序为Cu^<2+>>Fe^<3+>>Ni^<2+>>Mn^<2+>>Zn^<2+>.5. 质膜分离囊泡中微量元素吸收情况的检查在用几乎不缺铁的根部制备的分离的质膜囊泡的实验中，Cu或Fe的存在抑制了Cd的吸收，认为Cu的抑制作用大于Fe的抑制作用。归因于质膜囊泡的特征是铁和铜较少。