Evolution of fast skeletal myosin heavy chain genes of fish

鱼类快速骨骼肌球蛋白重链基因的进化

• 批准号: 23780214
• 负责人: IKEDA Daisuke
• 金额: $ 2.91万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780214/
• 关键词: ミオシン重鎖; ゲノム; 分子進化; 発生進化; ヤツメウナギ; ミオシン重鎖; ゲノム; 分子進化; 発生進化; ヤツメウナギ

In the present study, to elucidate the evolutionary relationship of fast skeletal myosin heavy chain genes (MYHs), MYHs of teleosts and tetrapods were compared with those of agnathan lampreys, one of the most primitive jawless fish. As a result, lampreys contained at least three fast skeletal MYHs which were clustered in ahead-to-tail manner on a single genomic region. Moreover, there was an apparent synteny in the corresponding MYH cluster regions between lamprey and tetrapod, implying that fast skeletal MYH clustering was a very early event in vertebrate evolution. Although zebrafish MYH promoters showed apparent activity to direct reporter gene expression in myogenic cells derived from mouse, those of lamprey had no activity. These results suggest thatthe common ancestor of vertebrate fast skeletal MYHs had been diverged into those of agnathan fish, teleosts and tetrapods. Tetrapods are assumed to have subsequently developed a transcriptional network system different from that of agnathan fish.

在本研究中，为了阐明快速骨骼肌球蛋白重链基因（MYHS）的进化关系，将硬骨鱼和四足动物的Myhs与Agnathan Lampreys的进化关系（MYHS）进行了比较，Agnathan Lampreys是最原始的无颌鱼之一。结果，lamp依至少包含三个快速骨骼MYH，这些MYH在单个基因组区域之前以前的方式聚集。此外，在七lamp和四足动物之间的相应MYH群集区域中存在明显的同步，这意味着快速骨骼MYH聚类是脊椎动物进化中非常早的事件。尽管斑马鱼MYH启动子在引导源自小鼠的肌源性细胞中表现出明显的活性，但lamprey的肌源性细胞没有活性。这些结果表明，脊椎动物快速骨骼myhs的共同祖先已经分歧为Agnathan鱼，硬骨鱼类和四足动物。假定四足动物随后开发了一种与Agnathan Fish不同的转录网络系统。