Chemical biological study of proanthocyanidins to elucidate the functionalities for human health

原花青素的化学生物学研究阐明其对人类健康的功能

• 批准号: 23780123
• 负责人: SAITH Akiko
• 金额: $ 3.16万
• 依托单位: Osaka Electro-Communication University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780123/
• 关键词: 生物活性物質; 化学生物学; proanthocyanidins; polyphenols; bioprobe; SAR; biological activity; chemical biology; proanthocyanidin; polyphenol; regioselective synthesis; 生物活性物質; 化学生物学; proanthocyanidins; polyphenols; bioprobe; SAR; biological activity; chemical biology; proanthocyanidin; polyphenol; regioselective synthesis

Proanthocyanidins (PAs), which have various strong biological activities, were modified with fluorescent molecules, fluorous tags, etc. by using our regioselective deprotection method for chemical biological studies of PAs. Using fluorous tags, the interaction between PAs and recombinant DNA polymerase ? was detected with SDS page. Furthermore, we identified that 3,3’’-digallate dimers showed extremely high activity against decrease in cell proliferation (HeLa S3). However, the effect of oligosaccharides for stabilization of PAs was not detected with antioxidant activity.

具有各种强大生物学活性的原蛋白素（PAS）通过使用我们的调节性脱位方法来修饰荧光分子，氟标签等。使用流畅的标签，PAS与重组DNA聚合酶之间的相互作用？用SDS页面检测到。此外，我们确定3,3'' - 化差二聚体对细胞增殖的下降表现出极高的活性（Hela S3）。然而，抗氧化活性未检测到寡糖对PAS稳定的影响。