Improvement of sensitivity for chemiluminescnt detection system using complex

使用复合物提高化学发光检测系统的灵敏度

基本信息

批准号：
18550073
负责人：
SAITO Keiitsu
金额：
$ 2.57万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

The chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) with various compounds was examined. Five-membered heteroaromatic compounds produced intense emission. The chemiluminescent intensity increased with an increase in electron density of the aromatic ring. Aliphatic secondary amines having a carboxyl group at the α-position showed very intense chemiluminescence. The chemiluminescent reaction of tris(2,2'-bipyridine)ruthenium(III) with 2,5-dimethylthiophene was examined in detail. Tris(2,21-bipyridine)ruthenium(III) reacted with 2,5-dimethylthiophene in the ratio of 2:1. The reaction product was identified as 5-methyl-2-thiophenecarbaldeyde. From the simultaneous measurement of the chemiluminescent intensity and the oxidation potential and previously reported studies on the oxidative reaction of thiophene derivatives, the chemiluminescent reaction mechanism involving deprotonation was estimated. Since imino acids in human body fluid are important biomakers for various ill … More nesses, a sensitive simultaneous determination method for imino acids in human serum was developed. Four imino acids were successfully determined within 60 min.To develop novel co-reactants for electrochemiluminesecence (ECL) immunoassays, preliminary studies on the tris(2,2'-bipyridine)ruthenium(II) ECL detection system using some compounds as co-reactants were performed. It was suggested that the sensitivity of the tris(2,2'-bipyridine)ruthenium(II)/ N,N-dipropyl-L-alanine system was comparable to that of the tris(2,2'-bipyridine)ruthenium(II)/ tripropylamine system, which has been widely used. Since N,N-dipropyl-L-alanine is a solid at ordinary temperature and its aqueous solution is neutral, it is ease of use compared to tripropylamine. A novel derivatization reagent, (2,4,5-trimethyl-3-thieny)acetic acid, for electrogenerated tris(2,2'-bipyridine)ruthenium (III) chemiluminescent detection was synthesized. Thirteen alkylphenols and bisphenol A in a mixed solution could be detected at ppb levels using high performance liquid chromatography. A sensitive determination method of phosphate in environmental water was developed. Molybdophosphate produced by the reaction of phosphate with molybdate in sulfuric acid formed an ion-pair with aliphatic tertiary amines. Phosphate was indirectly determined by the measurement of the aliphatic tertiary amines in the ion-pairs with tris(2,2'-bipyridine)ruthenium(III) chemiluminescent detection. Less

考察了三(2,2'-联吡啶)钌(III)与各种化合物的化学发光反应，其化学发光强度随着芳香族仲胺的电子密度的增加而增加。 α位具有羧基的化学发光反应非常强烈。详细研究了三(2,2'-联吡啶)钌(III)与2,5-二甲基噻吩的反应。三(2,21-联吡啶)钌(III)与2,5-二甲基噻吩以2:1的比例反应。通过同时测定化学发光强度和氧化电位，鉴定反应产物为5-甲基-2-噻吩甲醛。先前报道的噻吩衍生物氧化反应的研究，估计了涉及去质子化的化学发光反应机制。由于人体液中的亚氨基酸是多种疾病的重要生物制造者，因此开发了一种灵敏的人血清中亚氨基酸的同时测定方法。 60 分钟内成功测定四种亚氨基酸。为开发新型电化学发光（ECL）免疫分析共反应物，初步研究了以某些化合物为共反应物的三(2,2'-联吡啶)钌(II) ECL检测系统表明，三(2,2'-联吡啶)钌(II)/N的灵敏度较高。 N-二丙基-L-丙氨酸体系与三(2,2'-联吡啶)钌(II)/三丙胺体系相当，后者已被广泛使用。 N,N-二丙基-L-丙氨酸常温下为固体，水溶液呈中性，与三丙胺相比使用方便，是一种新型衍生化试剂（2,4,5-三甲基-3-噻吩基）乙酸。合成了十三烷基酚和双酚A，用于电化学发光检测三(2,2'-联吡啶)钌(III)。开发了一种灵敏测定环境水中磷酸盐的方法，该方法是由磷酸盐与硫酸中的钼酸盐与脂肪族叔胺形成离子对产生的。通过测量离子对中的脂肪族叔胺来间接测定磷酸盐三(2,2'-联吡啶)钌(III)化学发光检测。