Analysis for interaction between sweet receptor and taste modifiers

甜味受体与味觉调节剂之间的相互作用分析

• 批准号: 23792128
• 负责人: SANEMATSU Keisuke
• 金额: $ 2.58万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792128/
• 关键词: 味覚; 甘味受容体; 味覚修飾物質; グルマリン; ギムネマ酸; ミラクリン

Gymnemic acid (GA) and gurmarin (Gur) are known as selective inhibitors for sweet taste responses in mammals. To clarify the mechanisms of sweet-suppressing effects, we examined potential effects of these inhibitors on HEK293 cell responses heterologously expressed T1r2+T1r3. The results showed that similar to previous studies in humans and mice, GA and Gur inhibited the [Ca2+]i responses of HEK293 cells expressing human and mouse T1r2+T1r3 to various sweeteners and these effects were inhibited by γ- and β-CD, respectevely. We examined the responses of mouse/human chimeras of T1r2 and T1r3 to identify the interaction sites for GA and Gur. Our results suggest that the main target for GA’s action is transmembrane region of human T1r3 and Gur interacts with amino terminal region of mouse T1r3. Our mutation analysis indicate that the molecular basis for strain-specific sensitivity to Gur depends on a site within amino terminal region of mouse T1r2. In our models, GA is predicted to dock to a binding pocket within the transmembrane region of human T1r3 and Gur docks with amino terminal regions of mouse T1r2 and T1r3

体操酸（GA）被称为哺乳动物中甜味的选择性抑制剂。 ，GA和GUR抑制了表达人和MOUS E T1R2+T1R3对各种甜味剂的HEK293细胞的[Ca2+] I响应，并且通过γ抑制了ESE效应，我们检查了T1R2的小鼠/人嵌合体的响应，并识别相互作用位点的响应GA和GA的作用是人类T1R3的跨膜，而GUR与小鼠T1R3的氨基末端区域相互作用。在人类T1R3和GUR码头的跨膜内，具有小鼠T1R2和T1R3的氨基末端区域

项目成果

甘味抑制物質グルマリンの分子メカニズム

甜味抑制剂古马林的分子机制

• 发表时间: 2011
• 作者: 實松 敬介;二ノ宮 裕三
• 通讯作者: 二ノ宮 裕三

甘味受容体 T1r2/T1r3 に対する味覚修飾物質の相互作用部位の解析

味觉调节剂与甜味受体T1r2/T1r3相互作用位点分析

• 发表时间: 2011
• 作者: 實松 敬介;二ノ宮 裕三
• 通讯作者: 二ノ宮 裕三

中山書店

中山书店

• 发表时间: 2007
• 作者: 山崎 浩史;横井 毅;山崎 浩史
• 通讯作者: 山崎 浩史

甘味受容細胞の感受性抑制機構

甜味受体细胞的敏感性抑制机制

• 作者: 實松敬介;二ノ宮裕三
• 通讯作者: 二ノ宮裕三