Elucidating molecular basis of adult disease development by examining fetal tissues

通过检查胎儿组织阐明成人疾病发展的分子基础

• 批准号: 23591603
• 负责人: RAKWAL Randeep
• 金额: $ 3.24万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23591603/
• 关键词: Dohad; マイクロアレイ; プロテオーム; 脳; 低栄養; DOHaD; 胎児; 国際情報交換 アメリカ; 国際情報交流 フランス

Our results based on global gene expression analysis using DNA microarray revealed differential gene changes after maternal undernutrition, suggesting undernutrition influences mRNA expression of numerous genes including those that might be involved with neurodevelopmental disorders like autism or diabetes. Utilizing another complementary high-throughput omics approach, namely proteomics (2D-DIGE), we identified 15 differentially expressed proteins whose expression levels changed by undernutrition. Among 13 majorly down-regulated proteins, 5 proteins whose abundance decreased were identified as RNA interacting proteins, reported to be involved in enhancing stability of mRNA and accuracy of splicing.We believe that maternal undernutrition influenced mRNA stability and accuracy of splicing by a change in gene expressions along with decreased expression of RNA interacting protein in fetal brain, and which might result in psychiatric disorders and lifestyle-related diseases.

我们的结果基于使用DNA微阵列的全球基因表达分析分析，揭示了母体不足后的基因变化，表明营养不良会影响许多基因的mRNA表达，包括可能涉及自闭症或糖尿病（例如糖尿病）的神经发育障碍的基因。利用另一种互补的高通量法方法，即蛋白质组学（2D-DIGE），我们确定了15种差异表达的蛋白质，其表达水平随营养不足而变化。在13个主要下调的蛋白质中，5种蛋白质的丰度降低为RNA相互作用蛋白质，据报道与增强mRNA的稳定性和剪接的准确性有关。我们相信，孕产妇的营养不足会影响MRNA的稳定性和基因表达降低的RNA相互作用蛋白质和蛋白质的表达，并在RNA相互作用的表达中，并在fteral rna的表达中及其在ft脑相互作用的脑膜表达下，并可能由RNA相互作用，并可能产生。与生活方式有关的疾病。