Effect of radiofrequency hyperthermia on pain thresholds in osteoarthritis

射频热疗对骨关节炎痛阈的影响

• 批准号: 23590723
• 负责人: TAKAHASHI Kenji
• 金额: $ 3.24万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590723/
• 关键词: 変形性関節症; 温熱療法; 動物モデル; ラジオ波; 国際情報交換; 米国; 温熱慮法; 変形性関節症; 温熱療法; 動物モデル; ラジオ波; 国際情報交換; 米国; 温熱慮法

The purpose of this study was to clarify the mechanism underlying the therapeutic effects of radiofrequency hyperthermia in naturally occurring OA of Hartley guinea pigs by investigating the level of autophagy. Thermotron-RF300, which can apply a 13.56 MHz radio frequency pulse to the animal knees was used to perform hyperthermia. The quantitative analysis of the distribution of positive cells showed that, in the normal cartilage, ULK1 and Beclin1 were highly expressed. Seven days after the exposure to the radiofrequency, the expression levels of ULK1 and Beclin1 were augmented in animals with moderate OA. The severity of cartilage degradation was significantly reduced in the radiofrequency-treated knees compared with the untreated left knees. These results suggest that radiofrequency hyperthermia can stimulate autophagy, which is reduced in OA cartilage. In addition, radiofrequency reduced the severity of spontaneous osteoarthritis.

这项研究的目的是通过研究自噬水平来阐明射频高温高温对天然发生的OA天然OA的治疗作用的机制。可以在动物膝盖上施加13.56 MHz射频脉冲的Thermotron-RF300进行高温。阳性细胞分布的定量分析表明，在正常软骨中，ULK1和BECLIN1高度表达。暴露于射频的七天后，在具有中等OA的动物中，ULK1和BECLIN1的表达水平得到了增强。与未经治疗的左膝盖相比，在射频治疗的膝盖中，软骨降解的严重程度显着降低。这些结果表明，射频高温可以刺激自噬，在OA软骨中降低。另外，射频减少了自发性骨关节炎的严重程度。