Role of associative fiber tracts on sulcal development of the cerebrum in ferrets

联合纤维束对雪貂大脑脑沟发育的作用

• 批准号: 23590223
• 负责人: SAWADA Kazuhiko
• 金额: $ 3.49万
• 依托单位: Tsukuba International University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590223/
• 关键词: 大脳; 進化; 系統発生; MRI; フェレット; volumetry; 脳溝; 脳回; volume-rendering; voxel-based morphometry; volume rendering; DTI

Male and female ferrets at postnatal days (PDs) 4 to 90 were perfused with 4% paraformaldehyde solution, and the brains were removed. The chronology of sulcal and gyral emergences were clarified using those brain samples by gross anatomic observations. T1W-MRI was further acquired from the fixed brain using a high spatial resolution 7-tesla MRI. T1W-MRI-based maximum intensity projection (MIP) could delineate functional cortical areas related to myeloarchitecure in 3D. Quantitative analysis of the sulcal infolding revealed a male-prominent posterior extension of neocortical and archicortical sulci with cortical expansion in the temporo-parieto-occipital region of the cerebrum. Fractional anisotropy was measured in the subcortical white matter of the sulcal and gyral regions of the ferret cerebra using diffusion tensor imaging (DTI). However, it was unclear a relation of sulcal development with the development of subcortical white matter bundles.

出生后第4至90天的雄性和雌性雪貂用4％多聚甲醛溶液灌注，并取出大脑。通过大体解剖观察，使用这些大脑样本阐明了脑沟和脑回出现的时间顺序。使用高空间分辨率 7 特斯拉 MRI 从固定大脑进一步获取 T1W-MRI。基于 T1W-MRI 的最大强度投影 (MIP) 可以在 3D 中描绘与骨髓结构相关的功能皮质区域。对脑沟内折的定量分析显示，新皮质和古皮质脑沟的男性突出的后部延伸，以及大脑颞顶枕区的皮质扩张。 使用弥散张量成像（DTI）测量雪貂大脑脑沟和回回区域的皮层下白质的分数各向异性。然而，尚不清楚脑沟发育与皮质下白质束发育的关系。

项目成果

Application of gyrification stages of primates to gyrencephalic non-primate mammal, ferret (Mustela putorius)

灵长类动物的回转阶段在环脑非灵长类哺乳动物雪貂（Mustela putorius）中的应用

• 发表时间: 2014
• 期刊: Advanced Studies in Biology
• 作者: Hitomi J;Isogai S;Kimura E;朝克 吉楽，青山 裕彦;Sawada K
• 通讯作者: Sawada K

Development of cerebral sulci and gyri in ferrets (Mustela putorius)

• DOI: 10.1111/j.1741-4520.2012.00372.x
• 发表时间: 2012-09-01
• 期刊: CONGENITAL ANOMALIES
• 影响因子: 1.3
• 作者: Sawada, Kazuhiko;Watanabe, Misaki
• 通讯作者: Watanabe, Misaki

Sexual dimorphism of cerebral morphology in ferrets analyzed by MRI volumetry

MRI体积法分析雪貂大脑形态的性别二态性

• 发表时间: 2013
• 作者: Sawada K;Horiuchi-Hirose M;Saito S;Aoki I
• 通讯作者: Aoki I

MRI-based morphometric characterizations of sexual dimorphism of the cerebrum of ferrets (Mustela putorius)

• DOI: 10.1016/j.neuroimage.2013.06.024
• 发表时间: 2013-12
• 期刊: NeuroImage
• 影响因子: 5.7
• 作者: K. Sawada;Miwa Horiuchi-Hirose;S. Saito;I. Aoki

フェレット大脳における脳溝・脳回の形成

雪貂大脑脑沟和脑回的形成

• 发表时间: 2012
• 作者: Morita S;Hourai A;Miyata S;范 海明，坂本 信之，青山 裕彦;澤田和彦
• 通讯作者: 澤田和彦