Role of associative fiber tracts on sulcal development of the cerebrum in ferrets

联合纤维束对雪貂大脑脑沟发育的作用

• 批准号: 23590223
• 负责人: SAWADA Kazuhiko
• 金额: $ 3.49万
• 依托单位: Tsukuba International University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590223/
• 关键词: 大脳; 進化; 系統発生; MRI; フェレット; volumetry; 脳溝; 脳回; volume-rendering; voxel-based morphometry; volume rendering; DTI; 大脳; 進化; 系統発生; MRI; フェレット; volumetry; 脳溝; 脳回; volume-rendering; voxel-based morphometry; volume rendering; DTI

Male and female ferrets at postnatal days (PDs) 4 to 90 were perfused with 4% paraformaldehyde solution, and the brains were removed. The chronology of sulcal and gyral emergences were clarified using those brain samples by gross anatomic observations. T1W-MRI was further acquired from the fixed brain using a high spatial resolution 7-tesla MRI. T1W-MRI-based maximum intensity projection (MIP) could delineate functional cortical areas related to myeloarchitecure in 3D. Quantitative analysis of the sulcal infolding revealed a male-prominent posterior extension of neocortical and archicortical sulci with cortical expansion in the temporo-parieto-occipital region of the cerebrum. Fractional anisotropy was measured in the subcortical white matter of the sulcal and gyral regions of the ferret cerebra using diffusion tensor imaging (DTI). However, it was unclear a relation of sulcal development with the development of subcortical white matter bundles.

雄性和雌性雪貂在产后（PDS）4至90灌注4％多聚甲醛溶液，并去除大脑。使用这些大脑样本通过总解剖学观察来阐明沟和陀螺出现的年代。使用高空间分辨率7-TESLA MRI从固定脑中进一步获取T1W-MRI。基于T1W-MRI的最大强度投影（MIP）可以描绘3D中与骨髓骨架有关的功能性皮质区域。对沟内外的定量分析表明，新皮质和考古硫的男性主要后延伸，并在大脑的颞叶枕骨区域内具有皮质膨胀。 使用扩散张量成像（DTI）测量了在雪甲甲甲烷的沟和陀螺区域的皮质下白质（DTI）中测量的分数各向异性。然而，目前尚不清楚硫磺发育与皮质下白质束的发展有关。