Lipopolysaccharide remodeling in bacterial adaptation to host

细菌适应宿主过程中的脂多糖重塑

• 批准号: 23590095
• 负责人: KAWASAKI Kiyoshi
• 金额: $ 3.33万
• 依托单位: Doshisha Women's College of Liberal Arts
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590095/
• 关键词: エンドトキシン; Toll-like receptor; 細菌; 外膜; リポ多糖; 宿主認識; リモデリング; 認識; エンドトキシン; Toll-like receptor; 細菌; 外膜; リポ多糖; 宿主認識; リモデリング; 認識

Lipid A deacylated by outer membrane enzyme LpxR is less active than unmodified lipid A, but more active than lipid A precursor purified from LpxM mutant. These results suggest that lipid A modification by LpxR is beneficial for bacterial adaptation to host.Outer membrane vesicle was prepared from E. coli W3110, and the structure of lipid A and its protein composition were examined. Specific lipid A modification was not observed. On the other hand, it was revealed that the outer membrane vescicle contained flagellin, a component of flagella. The ability to form flagella, including the synthesis of flagella proteins, affected the production of outer membrane vesicles.

脂质A由外膜酶LPXR脱酰基比未修饰的脂质A的活性较低，但比脂质A的活性比从LPXM突变体纯化的前体A更活跃。这些结果表明，LPXR修饰脂质对细菌适应宿主是有益的。膜囊泡是从大肠杆菌W3110制备的，并检查了脂质A的结构及其蛋白质组成。 特定的脂质未观察到修饰。另一方面，据揭示了外膜花瓶中含有鞭毛蛋白，这是鞭毛的成分。 形成鞭毛的能力，包括鞭毛蛋白的合成，影响了外膜囊泡的产生。