Effects of estrogen and phytoestrogen on myogenesis

雌激素和植物雌激素对肌生成的影响

• 批准号: 23580182
• 负责人: YAMAJI Ryoichi
• 金额: $ 3.49万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23580182/
• 关键词: 骨格筋; エストロゲン; エストロゲン受容体; ユビキチン化; 脱ユビキチン化; 植物エストロゲン; ダイゼイン; 筋形成; 脱ユビキチン化酵素; 骨格筋; エストロゲン; エストロゲン受容体; ユビキチン化; 脱ユビキチン化; 植物エストロゲン; ダイゼイン; 筋形成; 脱ユビキチン化酵素

Skeletal muscle is the most abundant tissue in the body. Body composition and body size are different between males and females. Sex hormones are thought to be responsible for gender-specific differences in skeletal muscle mass, but their specific effects remain poorly characterized. Estrogen receptor (ER) alpha and ERbeta have been identified in skeletal muscle. Although estrogens seem to act on skeletal muscle through ER isoforms, the roles of E2 and the two ER isoforms in skeletal muscle remain unclear. In this study, I demonstrate that E2 inhibits myogenic differentiation of mouse C2C12 myoblasts and mouse satellite cells and increases the expression of USP19 in skeletal muscle in vitro and in vivo. Furthermore, I demonstrate that USP19 is induced by the nuclear ERalpha and inhibits myogenesis by acting as a deubiquitinating enzyme. I also analyzed the roles of the two ERs and ERbeta agonist in USP19 expression.

骨骼肌是体内最丰富的组织。男性和女性之间的身体成分和身体大小不同。性激素被认为是骨骼肌质量中性别特异性差异的原因，但其特异性影响仍然很差。在骨骼肌中已经鉴定出雌激素受体（ER）α和Erbeta。尽管雌激素似乎通过ER同工型作用于骨骼肌，但E2和两个ER同工型在骨骼肌中的作用尚不清楚。在这项研究中，我证明E2抑制了小鼠C2C12成肌细胞和小鼠卫星细胞的肌源分化，并在体外和体内增加USP19的表达。此外，我证明了USP19是由核Eralpha诱导的，并通过充当去泛素化酶来抑制肌发生。我还分析了两个ERS和Erbeta激动剂在USP19表达中的作用。