The differential analyses of tRNA splicing processes among the three domains of life

生命三个领域tRNA剪接过程的差异分析

• 批准号: 23570208
• 负责人: YOKOGAWA Takashi
• 金额: $ 3.41万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23570208/
• 关键词: tRNA; tRNAリガーゼ; スプライシング; 古細菌; EndA; RtcB; イントロン; 低分子環状RNA; アーケオシン; ArcTGT; BHBモチーフ; tRNA; tRNAリガーゼ; スプライシング; 古細菌; EndA; RtcB; イントロン; 低分子環状RNA; アーケオシン; ArcTGT; BHBモチーフ

In Archaea and Eucarya, some tRNA genes contain introns. Since the precursor tRNA (pre-tRNA) is not functional in protein biosynthesis, exact removal of introns must be essential. Recently, it was found that the RtcB catalyzes the ligation of exons derived from the pre-tRNA. RtcB also seems to catalyze the circulation of intron from pre-tRNA. In fact, we found the circularized intron in methanogenic archaeon, Methanosarcina acetivorans, cells. Whereas the circularized intron has any function or not, we believe it would be functional because of the following facts. First, the circularized intron was stable in M. acetivorans cells. Second, RtcB even exists in bacterial cells that any tRNA gene does not contain intron. In future, we would like to discover the functions of small circularized RNAs.

在古细菌和桉树中，一些tRNA基因包含内含子。由于前体tRNA（pre-tRNA）在蛋白质的生物合成中不起作用，因此内含子的精确去除必须是必不可少的。最近，发现RTCB催化了源自pre-tRNA的外显子的连接。 RTCB似乎也催化了pre-tRNA内含子的循环。实际上，我们发现了甲烷元素古代，甲氧骨乙酸酯，细胞中的圆形内含子。尽管循环内含子具有任何功能，但我们认为由于以下事实，它将起作用。首先，循环内含子在乙酰基菌细胞中稳定。其次，RTCB甚至存在于细菌细胞中，任何tRNA基因都不包含内含子。将来，我们想发现小圆形RNA的功能。