Availability of MRI and MRS for talent identification in terms of muscle properties.

MRI 和 MRS 可用于肌肉特性方面的人才识别。

基本信息

批准号：
17300214
负责人：
TAKAHASHI Hideyuki
金额：
$ 8.01万
依托单位：
National Agency for the Advancement of Sports and Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300214/
关键词：
skeletal muscle magnetic resonance imaging magnetic resonance spectroscopy muscle fiber composition oxidative capacity talent identification diffusion glycogen 循環系能力

项目摘要

It is necessary to identify effectually and properly individuals who are endowed with outstanding sports capabilities in order to improve Japan's international competitiveness in sports. Talent identification in terms of skeletal muscle is especially important because the muscle performance affects sports performance. This study investigated the availability of magnetic resonance imaging(MRI) and magnetic resonance spectroscopy(MRS) for evaluation of muscle properties in order to identify individuals with a talent for sports.We performed MRI, phosphorus (^<31>P)- and carbon (^<13>C)-MRS, and biochemical assay of the skeletal muscle in volunteers. There were significant correlations between phosphocreatine (PC) / adenosine triphosphate (ATP), (PCr + inorganic phosphate (Pi) ) /ATP, transverse relaxation time (T2) and muscle fiber type composition. In the experiment comparing MRI and ^<31>P-MRS data between sprinters and endurance runners, the former showed significantly higher PCr/ATP, (PCr+Pi)/ATP, intracellular pH and lower T2 than the latter. We demonstrated that ^<13>C-MRS using normal 1.5-tesla clinical scanner enables noninvasive measurement of the muscle glycogen concentration. Moreover, we found an optimal condition for evaluating the diffusion capacity of skeletal muscle, which distinguished muscles that have a distinct diffusion property.To investigate muscle energetics during exercise, we performed ^<31>P-MRS and near infrared spectroscopy simultaneously and demonstrated that muscles possessing higher oxidative capacity show superior oxygen availability.These findings suggest that MRI and MRS can noninvasively evaluate the fiber type composition, concentration of energy substrates, microcirculation and oxidative capacity in human skeletal muscle.

为了提高日本体育的国际竞争力，有必要有效、适当地甄别具有杰出体育能力的个人。骨骼肌方面的人才鉴定尤为重要，因为肌肉性能影响运动表现。本研究调查了磁共振成像 (MRI) 和磁共振波谱 (MRS) 用于评估肌肉特性的可用性，以便识别具有运动天赋的个体。我们进行了 MRI、磷 (^<31>P)- 和碳检查(^ 13 C)-MRS和志愿者骨骼肌的生化测定。磷酸肌酸（PC）/三磷酸腺苷（ATP）、（PCr+无机磷酸盐（Pi））/ATP、横向弛豫时间（T2）与肌纤维类型组成之间存在显着相关性。在比较短跑运动员和耐力跑运动员之间的MRI和^ 31 P-MRS数据的实验中，前者显示出明显高于后者的PCr/ATP、(PCr+Pi)/ATP、细胞内pH和较低的T2。我们证明，使用正常1.5特斯拉临床扫描仪的13 C-MRS能够实现肌糖原浓度的无创测量。此外，我们找到了评估骨骼肌扩散能力的最佳条件，该条件可以区分具有独特扩散特性的肌肉。为了研究运动期间的肌肉能量学，我们同时进行了^<31>P-MRS和近红外光谱分析，并证明了具有较高氧化能力的肌肉显示出优越的氧气利用率。这些研究结果表明，MRI 和 MRS 可以无创地评估人体骨骼肌的纤维类型组成、能量底物浓度、微循环和氧化能力。