Experiment research on the therapeutic impact of low-magnitude vibrations on eraly-stage bone healing

低强度振动对早期骨愈合治疗影响的实验研究

• 批准号: 23300165
• 负责人: MATSUMOTO Takeshi
• 金额: $ 11.15万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23300165/
• 关键词: 骨折修復; 血管新生; 高周波微振動; 放射光CT; 骨修復; 全身性微振動; 低酸素誘導因子; 全身性高周波微振動; 骨再生; コラーゲン; 第2高調波発生光; 高解像度CT

Promotion of bone healing is favorable for preventing the elderly from falling into osteoporosis, a bedridden state, or situation necessitating nursing care. This study was undertaken to evaluate the effect of low-magnitude whole-body vibration (WBV) on early-stage bone healing, mainly focusing on angiogenesis. Mice subjected to a drill-hole surgery on a tibial diaphysis were exposed to 0.3g WBV at 30 Hz for 20 min/day over a 12-day period. Following vascular casting, the defect site was CT-scanned by synchrotron light. Structural analysis suggests the WBV-induced promotion of the defect repair with reduced angiogenesis. In addition, the second harmonic generation microscopy showed that WBV had no effect on collagen synthesis. Furthermore, the enhancement of angiogenesis under WBV did not increase the promotion effect of WBV on bone healing.

促进骨骼愈合，有利于防止老年人出现骨质疏松、卧床不起或需要护理的情况。本研究旨在评估低强度全身振动（WBV）对早期骨愈合的影响，主要关注血管生成。对胫骨骨干进行钻孔手术的小鼠在 12 天的时间内暴露于 30 Hz 的 0.3 g WBV，每天 20 分钟。血管铸型后，用同步加速器光对缺损部位进行 CT 扫描。结构分析表明，WBV 诱导促进缺损修复并减少血管生成。此外，二次谐波显微镜显示WBV对胶原蛋白合成没有影响。此外，WBV下血管生成的增强并没有增加WBV对骨愈合的促进作用。

项目成果

サブトラクション放射光CTによる骨欠損修復早期の骨再生及び血管新生定量イメージング

减影同步辐射CT对早期骨缺损修复过程中骨再生和血管生成的定量成像

• 发表时间: 2011
• 作者: 後藤大智;松本健志;内藤尚;田中正夫
• 通讯作者: 田中正夫

Quantitative Bone CT Using Synchrotron Source

使用同步加速器源的定量骨 CT

• 发表时间: 2013
• 作者: 桝屋啓志;田中信彦;鈴木智広;若菜茂晴;T. Matsumoto
• 通讯作者: T. Matsumoto

Subtraction micro-computed tomography of angiogenesis and osteogenesis during bone repair using synchrotron radiation with a novel contrast agent

• DOI: 10.1038/labinvest.2013.87
• 发表时间: 2013-09-01
• 期刊: LABORATORY INVESTIGATION
• 影响因子: 5
• 作者: Matsumoto, Takeshi;Goto, Daichi;Sato, Syota
• 通讯作者: Sato, Syota

In vivo CT quantification of trabecular bone dynamics in mice after sciatic neurectomy using monochromatic synchrotron radiation

使用单色同步辐射对坐骨神经切除术后小鼠骨小梁动力学进行体内 CT 量化

• 发表时间: 2011
• 期刊: Calcif.Tissue Int.
• 作者: Takeshi Matsumoto;et al.
• 通讯作者: et al.

Second Harmonic Generation Microscopy and Synchrotron Radiation CT for the Determination of Collagen and Mineral Deposition in Early-Stage Bone Repair: Effect of Whole Body Micro-Vibration

二次谐波显微镜和同步辐射 CT 测定早期骨修复中的胶原蛋白和矿物质沉积：全身微振动的影响

• 发表时间: 2014
• 期刊: Adv Biomed Eng
• 影响因子: 1
• 作者: Y. Hashimoto;S. Fukushima;T. Matsumoto;H. Naito;M. Tanaka
• 通讯作者: M. Tanaka