Microirradiation system for centrosome in human-derived living cells

人源活细胞中心体微辐射系统

• 批准号: 22740269
• 负责人: MEISSL WALTER
• 金额: $ 2.83万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22740269/
• 关键词: ビーム物理; マイクロビーム; ガラスキャピラリー; 生物物理

A fully automatic 3-D target stage for the microirradiation of living cells was successfully acquired. We can thus visit any cell within the target zone of a cell dish and position target any point within the cell precisely. We could, for example, add an algorithm to identify cells automatically or integrate the ability to get so called z-stack images which allow a 3-dimensional representation of cells and areas affected by irradiation. First tests of the system show that all components are fully working and that the desired sub-micrometer precision is reached in all three dimensions. Living human cells kept in the on-stage incubator for several days remained healthy over the full period and the cell cycle of the cells was measured as less than 20 hours. In a first test with fixed(not moving) cells we were able to precisely aim the ion beam at the circa 1 micrometer wide centrosome, an organelle within the cells. The improved system will allow precise targeting of sub-cellular components as well as tracking of irradiated cells for several days without any harmful effects on the cells.

成功获得了活细胞微辐照的全自动3-D目标阶段。因此，我们可以访问细胞盘目标区域内的任何单元，并将其定位为目标。例如，我们可以添加一种算法来自动识别单元格或整合获得所谓的Z-stack图像的能力，该图像允许3维表示细胞和受辐射影响的区域。该系统的首次测试表明，所有组件都完全有效，并且在所有三个维度上都达到了所需的子微米精度。活着的人类细胞在整个时期内保持在舞台上的孵化器几天保持健康，并且细胞周期的测量小于20小时。在对固定（不动）细胞的第一次测试中，我们能够将离子束准确地瞄准大约1微米宽的中心体，即细胞内的细胞器。改进的系统将允许精确靶向亚细胞成分以及对辐照细胞的跟踪几天，而对细胞没有任何有害影响。

项目成果

Closed-tip tapered glass capillaries as a novel tool for micro-irradiation of living cells by MeV ion beams(invited progress reprt)

闭尖锥形玻璃毛细管作为 MeV 离子束微照射活细胞的新型工具（特邀进展报告）

• 发表时间: 2010
• 作者: 高橋聡;高塚和夫;Walter Meissl
• 通讯作者: Walter Meissl

Three-dimensional micro-irradiation of living cells with ion beams in vivo and in vitro

离子束体内外活细胞三维微照射

• 发表时间: 2011
• 作者: Katsumasa Kamiya;Naoto Umezawa;Susumu Okada;MEISSL WALTER;岩間隼;川口 由紀;高橋聡;MEISSL WALTER
• 通讯作者: MEISSL WALTER

First results from micro-irradiation of living cells by tapered glass capillaries

锥形玻璃毛细管对活细胞进行微照射的第一个结果

• 发表时间: 2010
• 作者: Nguyen Thanh Phuc;川口由紀;上田正仁;Walter Meissl
• 通讯作者: Walter Meissl

Application of insulating nanocapillaries in living human cell irradiation

绝缘纳米毛细管在人体活细胞辐照中的应用

• 发表时间: 2011
• 作者: Katsumasa Kamiya;Naoto Umezawa;Susumu Okada;MEISSL WALTER
• 通讯作者: MEISSL WALTER

Closed-tip tapered glass capillaries as a novel tool for micro-irradiation of living cells by MeV ion beams

封闭尖端锥形玻璃毛细管作为 MeV 离子束微照射活细胞的新型工具

• 发表时间: 2010
• 作者: Katsumasa Kamiyazawa;Naoto Umezawa;Susumu Okada;Walter Meissl
• 通讯作者: Walter Meissl