Elucidation of the functional role of excitatory and inhibitory inputs in output station of basal ganglia.

阐明基底神经节输出站兴奋性和抑制性输入的功能作用。

• 批准号: 22700420
• 负责人: HATANAKA Nobuhiko
• 金额: $ 2.58万
• 依托单位: National Institute for Physiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22700420/
• 关键词: 姿勢・運動制御; 大脳基底核; マカクサル; 視床下核; 淡蒼球内節; 淡蒼球; 線条体

The activity of the output station of basal ganglia is regulated with the timing and balance of two competitive inputs, i.e. excitatory input from subthalamic nucleus and inhibitory input from striatum. Using macaque monkeys, single unit activity changes during hand reaching task with delay were recorded in internal segment of globus pallidus (GPi). The confirmation of cortical information source were carried out by the multi-synaptic orthodromic response to electrical stimulation of primary motor cortex and supplementary motor area that identified by ICMS. Thereafter, neuropharmacological blocking of excitatory or inhibitory receptor was performed by micro-drug-injection in vicinity of recording neuron. Drugs were Gabazine (GABAA receptor antagonist) and cocktail of CPP (NMDA receptor antagonist) and NBQX (AMPA receptor antagonist) and their concentrations were 0.5 . 1 mM. Among 52 GPi recording neurons with receptor blocking, all neurons showed activity changes according to each inputs. Some neurons that its activity increased during movement showed robust inhibitory response after glutamatergic receptor blocking. This result suggest that GPi neurons regulated by not only inhibitory input from striatum but also excitatory subthalamic input.

基底神经节输出站的活动受到两种竞争性输入的时间和平衡的调节，即来自丘脑底核的兴奋性输入和来自纹状体的抑制性输入。使用猕猴，在苍白球内段（GPi）记录了手伸任务期间延迟的单个单位活动变化。通过ICMS识别的初级运动皮层和辅助运动区的多突触顺向电刺激反应来确认皮质信息源。此后，通过在记录神经元附近注射微量药物来进行兴奋性或抑制性受体的神经药理学阻断。药物为加巴嗪(GABAA受体拮抗剂)和CPP(NMDA受体拮抗剂)和NBQX(AMPA受体拮抗剂)的混合物，浓度为0.5。 1毫米。在受体阻断的 52 个 GPi 记录神经元中，所有神经元都根据每次输入显示出活动变化。一些在运动过程中活性增加的神经元在谷氨酸受体阻断后表现出强烈的抑制反应。这一结果表明，GPi 神经元不仅受到纹状体的抑制性输入的调节，还受到兴奋性底丘脑输入的调节。

项目成果

運動課題遂行中のサルにおける淡蒼球ニューロン活動のグルタミン酸およびGABA作動性調節

运动任务执行过程中猴子苍白球神经元活动的谷氨酸和 GABA 能调节

• 作者: Tohru Takemasa;Yousuke Abe;Chiaki Kumagai;Hidenori Kiyosawa;松元剛;Yasuto Kobayashi,Michiyoshi Ae,Akiyo Miyazaki,Norihisa Fujii;福村龍太郎;畑中伸彦，高良沙幸，金子将也，南部 篤
• 通讯作者: 畑中伸彦，高良沙幸，金子将也，南部 篤

Differential activity patterns of putaminal neurons with inputs from the primary motor cortex and supplementary motor area in behaving monkeys

行为猴初级运动皮层和辅助运动区输入的壳核神经元的差异活动模式

• 发表时间: 2011
• 期刊: J Neurophysiol
• 影响因子: 2.5
• 作者: Takara S;Hatanaka N;Takada M;Nambu A
• 通讯作者: Nambu A

Glutamatergic and GABAergic modulation of monkey pallidal neuron activity in relation to motor task

与运动任务相关的猴苍白球神经元活动的谷氨酸和 GABA 能调节

• 发表时间: 2011
• 作者: Hatanaka N;Kaneko N;Takara S;Takada M;Nambu A
• 通讯作者: Nambu A

Flavoprotein fluorescence imaging of motor cortical areas in a macaque monkey

猕猴运动皮质区域的黄素蛋白荧光成像

• 发表时间: 2012
• 作者: Hatanaka N;Kaneko N;Takara S;Nambu A
• 通讯作者: Nambu A