Integrated in vit-roin vivo organization of liver tissues having a functional bile duct network

具有功能性胆管网络的肝组织的体外整合组织

• 批准号: 22650108
• 负责人: SAKAI Yasuyuki
• 金额: $ 2.2万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22650108/
• 关键词: 細胞・組織工学; マクロ組織極性; 移植・再生医療; 再生医学; 細胞・組織; 生物・生体工学; 肝細胞; 胆管上皮細胞; 球状凝集体; 胆管; 組織工学; 極性; スフェロイド; 胆管ネットワーク

To organize implantable liver tissues having a functional bile duct network, first, we made spherical cellular aggregates constituted of fetal rat liver cells and mature bile duct epithelial cells isolated from rats. Aggregates having a continuous and functional bile duct network over the entire aggregates were successfully formed in the aggregates containing a three times larger number of mature bile duct cells over fetal liver cells. When such aggregates were immobilized in poly-L-lactic acid scaffolds with Matrigel and were implanted into the mesentery of immune-deficient mice, bile duct networks were well maintained in the case of aggregates having mature bile duct cells, whereas they were not formed in the case of pure fetal liver cell aggregates. These results showed that mature bile duct cells are necessary in forming functional bile duct networks over organized liver tissues.

为了组织具有功能性胆管网络的可植入肝组织，首先，我们使球形细胞聚集体由胎儿大鼠肝细胞和从大鼠分离的成熟胆管上皮细胞组成。在整个聚集体上具有连续且功能性的胆管网络的聚集体成功地形成了包含胎儿肝细胞上大量成熟的胆管细胞的三倍的聚集体。当将这种聚集体固定在多l-乳酸酸支架中，并将其植入免疫缺陷小鼠的肠系膜中时，在具有成熟的胆管细胞的聚集体的情况下，胆管网络得到很好的维持，而在纯胎儿肝细胞的纯乳细胞聚集体的情况下，它们没有形成。这些结果表明，在有组织的肝组织上形成功能性胆管网络时，成熟的胆管细胞是必需的。

项目成果

異所性体内埋め込み型人工肝臓開発の現状と展望

异位植入式人工肝的发展现状及前景

• 发表时间: 2010
• 期刊: 生産研究
• 作者: 勝田毅;小森喜久夫;寺谷工;落谷孝広;酒井康行
• 通讯作者: 酒井康行

間葉系幹細胞を用いた細胞治療の可能性

使用间充质干细胞进行细胞治疗的可能性

• 发表时间: 2010
• 期刊: 医工学治療
• 作者: 勝田毅;酒井康行;落谷孝広
• 通讯作者: 落谷孝広

Hypoxia efficiently induces differentiation of mouse embryonic stem cells into endodermal and hepatic progenitor cells

• DOI: 10.1016/j.bej.2013.02.012
• 发表时间: 2013-05-15
• 期刊: BIOCHEMICAL ENGINEERING JOURNAL
• 影响因子: 3.9
• 作者: Katsuda, Takeshi;Teratani, Takumi;Sakai, Yasuyuki
• 通讯作者: Sakai, Yasuyuki

Fetal Tissue Transplantation in Fetal Tissue Transplantation（分担執筆）,

胎儿组织移植中的胎儿组织移植（合著者），

• 发表时间: 2013
• 作者: Y. Sakai1;J. Jiang;S. Hanada;H. Huang;T. Katsuda;N. Kojima;T. Teratani;A. Miyajima;T. Ochiya
• 通讯作者: T. Ochiya