Pre- and post-synaptic cell type dependent propofol effects on GABAergic synaptic transmission in rat insular cortex.

突触前和突触后细胞类型依赖性丙泊酚对大鼠岛叶皮质 GABA 能突触传递的影响。

• 批准号: 22592264
• 负责人: OI Yoshiyuki
• 金额: $ 2.75万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592264/
• 关键词: 歯科麻酔学; プロポフォール; 大脳皮質; 抑制性シナプス後電流; propofol; pain; insular cortex; GABA(A) receptor; IPSC; whole-cell patch-clamp; GABA; patch clamp

To examine whether propofol effects on unitary inhibitory postsynaptic currents (uIPSCs) are differentially regulated depending on their pre- and postsynaptic cell types, we performed multiple whole-cell patch-clamp recording from layer V GABAergic interneurons and pyramidal (Pyr) cells of rat insular cortex (IC). Comparing the presynaptic FS and presynaptic non-FS connections, the presynaptic FS connections showed larger enhancement of uIPSC charge transfer by propofol. Among the presynaptic FS connections, FS→Pyr connections showed larger enhancement of uIPSC charge transfer thanFS→interneurons, indicating that the primary target of propofol to GABAergic synaptic transmission is FS→Pyr connections.

为了检查异丙酚对单一抑制性突触后电流 (uIPSC) 的影响是否根据突触前和突触后细胞类型的不同而受到不同的调节，我们对大鼠岛叶的 V 层 GABA 能中间神经元和锥体 (Pyr) 细胞进行了多次全细胞膜片钳记录。比较突触前 FS 和突触前非 FS 连接，突触前 FS 连接显示出更大的增强。在突触前 FS 连接中，FS→Pyr 连接显示出比 FS→中间神经元更大的 uIPSC 电荷转移增强，表明异丙酚到 GABA 能突触传递的主要目标是 FS→Pyr 连接。

项目成果

Anatomical and electrophysiological mechanisms for asymmetrical excitatory propagation in the rat insular cortex: In vivo optical imaging and whole-cell patch-clamp studies

• DOI: 10.1002/cne.23246
• 发表时间: 2013-05-01
• 期刊: JOURNAL OF COMPARATIVE NEUROLOGY
• 影响因子: 2.5
• 作者: Adachi, Kazunori;Fujita, Satoshi;Kobayashi, Masayuki
• 通讯作者: Kobayashi, Masayuki

Responses of insular cortical neurons to orofacial noxious stimulation

岛叶皮质神经元对口面部伤害性刺激的反应

• 发表时间: 2012
• 作者: Adachi K;Beppu Y;Koshikawa N;Sakagami H;Shimada J;Kobayashi M
• 通讯作者: Kobayashi M

島皮質AIの舌侵害刺激への応答

岛叶皮质AI对舌头伤害性刺激的反应

• 发表时间: 2011
• 作者: Nakayama K;Mochizuki A;Nakamura S;Inoue T;Haque T;安達一典
• 通讯作者: 安達一典

Gustatory imagery reveals functional connectivity from the prefrontal to insular cortices traced with magnetoencephalography.

• DOI: 10.1371/journal.pone.0021736
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Kobayashi M;Sasabe T;Shigihara Y;Tanaka M;Watanabe Y
• 通讯作者: Watanabe Y

味覚想起課題において前頭前野は島皮質に先行して活性化される

在味觉回忆任务中，前额皮质先于岛叶皮质被激活。

• 发表时间: 2010
• 作者: 小林真之;越川憲明
• 通讯作者: 越川憲明