Pre- and post-synaptic cell type dependent propofol effects on GABAergic synaptic transmission in rat insular cortex.

突触前和突触后细胞类型依赖性丙泊酚对大鼠岛叶皮质 GABA 能突触传递的影响。

• 批准号: 22592264
• 负责人: OI Yoshiyuki
• 金额: $ 2.75万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592264/
• 关键词: 歯科麻酔学; プロポフォール; 大脳皮質; 抑制性シナプス後電流; propofol; pain; insular cortex; GABA(A) receptor; IPSC; whole-cell patch-clamp; GABA; patch clamp; 歯科麻酔学; プロポフォール; 大脳皮質; 抑制性シナプス後電流; propofol; pain; insular cortex; GABA(A) receptor; IPSC; whole-cell patch-clamp; GABA; patch clamp

To examine whether propofol effects on unitary inhibitory postsynaptic currents (uIPSCs) are differentially regulated depending on their pre- and postsynaptic cell types, we performed multiple whole-cell patch-clamp recording from layer V GABAergic interneurons and pyramidal (Pyr) cells of rat insular cortex (IC). Comparing the presynaptic FS and presynaptic non-FS connections, the presynaptic FS connections showed larger enhancement of uIPSC charge transfer by propofol. Among the presynaptic FS connections, FS→Pyr connections showed larger enhancement of uIPSC charge transfer thanFS→interneurons, indicating that the primary target of propofol to GABAergic synaptic transmission is FS→Pyr connections.

为了检查对单一抑制性突触后电流（UIPSC）的影响是否根据其前和突触后细胞类型的不同，我们进行了多个来自V层的全细胞斑块钳记录，该层从V层GABA能中神经元和大鼠Insular Insular Cortex（IC）的Pyramidal（Pyr）细胞进行了多次记录。比较突触前的FS和突触前的非FS连接，突触前FS连接显示了通过提案对UIPSC电荷转移的更大增强。在突触前的FS连接中，FS→PYR连接显示了UIPSC电荷转移的增强，而不是FFS→中间神经元，这表明gabaergic Synaptic Transmins的提案的主要目标是FS→Pyr Connections。