Mutagenesis analysis of hydrogen bond network formed in bovine heart cytochrome c oxidase

牛心脏细胞色素c氧化酶形成的氢键网络的诱变分析

• 批准号: 22770155
• 负责人: AMINAKA Ryohta
• 金额: $ 2.75万
• 依托单位: University of Hyogo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22770155/
• 关键词: タンパク質; 核酸の構造; 動態; 機能; 酵素; 核酸; チトクロムc酸化酵素

We carried out mutagenesis analyses of a hydrogen bond network, designated as D-pathway, formed in the bovine heart cytochrome c oxidase employing HeLa cell bovine/human hybrid enzyme expression system developed by us. A pathway corresponding to this D-pathway is also found in the bacterial enzymes, and has been proposed to convey both pumping and water forming protons. The structures of these two pathways are almost identical to each other and amino acid residues essential to the bacterial enzymes are conserved in the bovine enzyme. Hence, it has been thought that these two have the same function, thereby opposing to the proton-pumping pathway of the bovine enzyme proposed by us. The results of this study strongly suggest D-pathway is not for pumping proton but for water forming proton.

我们对使用HELA细胞牛/人类杂化酶表达系统的牛心脏细胞色素C氧化酶形成的氢键网络进行了诱变分析，该氢键分析是在牛心脏细胞色素C氧化酶中形成的。在细菌酶中也发现了与该D径道相对应的途径，并已提出同时传达泵送和水形成质子。这两种途径的结构几乎相同，而细菌酶必不可少的氨基酸残基在牛酶中保守。因此，人们认为这两个功能具有相同的功能，从而与我们提出的牛酶的质子泵送途径相反。这项研究的结果强烈表明D-Pathway不是用于泵送质子，而是用于形成质子的水。

项目成果

Mutagenesis studies on D-pathway function of bovine heart cytochrome c oxidase

牛心细胞色素c氧化酶D通路功能的诱变研究

• 发表时间: 2010
• 期刊: Biochimica Biophysica Acta
• 作者: Ryohta Aminaka;Kunitoshi Shimokata;Mai Itoh;Yukie Katayama;Tomitake Tsukihara;Shinya Yoshikawa;and Hideo Shimada
• 通讯作者: and Hideo Shimada

Characterization by mutagenesis analysis of putative proton transfer pathway, D-pathway of bovine heart cytochrome c oxidase

牛心细胞色素 c 氧化酶的假定质子转移途径、D 途径的诱变分析表征

• 发表时间: 2012
• 期刊: 生物物理
• 作者: Ryohta Aminaka;Kunitoshi Shimokata;Mai Itoh;Yukie Katayama;Tomitake Tsukihara;Shinya Yoshikawa;and Hideo Shimada
• 通讯作者: and Hideo Shimada