A search for the interaction molecules with REIC/Dkk-3 in prostate gland.

前列腺中 REIC/Dkk-3 相互作用分子的研究。

• 批准号: 22791476
• 负责人: OCHIAI Kazuhiko
• 金额: $ 2.5万
• 依托单位: Nippon Veterinary and Life Science University (2011)The University of Tokushima (2010)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791476/
• 关键词: 癌; 遺伝子; REIC/Dkk-3; REIC; Dkk-3; 前立腺がん; 相互作用; Tctex-1; Dkk3

REIC/Dkk-3 acts as a tumor suppressor in multiple cancer cell lines by inducing apoptosis through endoplasmic reticulum(ER) stress signaling. However, the intracellular interaction partners of REIC/Dkk-3 have not been fully elucidated. By employing yeast two-hybrid screening, we identified the human dynein light chain, Tctex-1, as a novel interaction partner of REIC/Dkk-3.The binding region of REIC/Dkk-3 with Tctex-1 contains an amino acid sequence motif [-E-X-G-R-R-X-H-] which was previously reported as the Tctex-1 binding domain of dynein intermediate chain(DIC). The link between REIC/Dkk-3 and Tctex-1 may be of significance for understanding the molecular functions of the proteins in ER stress signaling and intracellular dynein motor dynamics, respectively.

REIC/DKK-3通过通过内质网（ER）应力信号传导诱导多个癌细胞系中的肿瘤抑制剂。但是，REIC/DKK-3的细胞内相互作用伙伴尚未完全阐明。 By employing yeast two-hybrid screening, we identified the human dynein light chain, Tctex-1, as a novel interaction partner of REIC/Dkk-3.The binding region of REIC/Dkk-3 with Tctex-1 contains an amino acid sequence motif [-E-X-G-R-R-X-H-] which was previously reported as the Tctex-1 binding domain of dynein intermediate chain(DIC). REIC/DKK-3和TCTEX-1之间的联系对于理解蛋白质在ER应力信号传导和细胞内动力蛋白运动动力学中的分子功能可能具有重要意义。

项目成果

Interactions between canine RAD51 and full length or truncated BRCA2 BRC repeats

• DOI: 10.1016/j.tvjl.2010.11.001
• 发表时间: 2011-11-01
• 期刊: VETERINARY JOURNAL
• 影响因子: 2.2
• 作者: Ochiai, K.;Yoshikawa, Y.;Morimatsu, M.
• 通讯作者: Morimatsu, M.

REIC/Dkk-3タンパク質の部分領域ポリペプチド

REIC/Dkk-3蛋白部分区多肽

• 发表时间: 2011

癌抑制遺伝子BRCA2のPCR法による変異解析法の確立

抑癌基因BRCA2 PCR法突变分析方法的建立

• 发表时间: 2011
• 作者: 吉永泰永・森松正美・新井敏郎;他
• 通讯作者: 他

Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51

• DOI: 10.1016/j.febslet.2011.05.027
• 发表时间: 2011-06-23
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Ochiai, Kazuhiko;Yoshikawa, Yasunaga;Morimatsu, Masami
• 通讯作者: Morimatsu, Masami

イヌにおけるヒトABO組織血液型遺伝子に相同なfull length cDNAの同定と特徴

犬人 ABO 血型基因同源全长 cDNA 的鉴定和表征

• 发表时间: 2011
• 作者: 近江俊徳;鄭英和;市東正幸;宇田川智野;河上剛;落合和彦;杉山将;土田修一
• 通讯作者: 土田修一