Analysis of infectious tolerance in chronic middle ear inflammation with nontypeable Haemophilus influenzae

未分型流感嗜血杆菌慢性中耳炎感染耐受性分析

• 批准号: 22591885
• 负责人: HIRANO Takashi
• 金额: $ 1.83万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591885/
• 关键词: 中耳; 慢性炎症; インフルエンザ菌; T 細胞; 粘膜免疫; 中耳炎; Th17細胞; 制御性T細胞; 自然免疫; 慢性中耳炎; 慢性感染

Nontypeable Haemophilus influenzae (NTHi) is considered a major pathogen underlying middle ear infection. This study aimed to investigate the dynamics of IL-17 in chronic otitis media induced by NTHi in Balb/c mice. NTHi was injected into the tympanic bulla with Eustachian tubal obstruction. Middle ear effusions and tissues were collected at day 3, 14, 2 month after injection. The concentration of IL-17 in middle ear effusions eleveated significantly during experiments when compared to control. IL-17 producing ・・ T cells and Th17 cells were also increased up to 6 months in the middle ear. Based on these findings, IL-17 producing T cells existed in chronic state of otitis media, and may play some role for modification of chronic inflammation in the middle ear.

不可用的嗜血杆菌影响（NTHI）被认为是中耳感染的主要病原体。这项研究旨在研究BALB/C小鼠NTHI诱导的慢性中耳炎中IL-17的动力学。 NTHI被注入了带有尤斯塔克式输卵管对象的鼓膜。注射后第3、14，第3，14天收集中耳积液和组织。与对照相比，在实验期间，中耳积液中IL-17的浓度显着升高。 IL-17产生... T细胞和Th17细胞在中耳中也增加了6个月。基于这些发现，IL-17产生的T细胞存在于中耳炎的慢性状态，可能在中耳中慢性炎症中起作用。

项目成果

Effect of low-intensity focused ultrasound on the middle ear in a mouse model of acute otitis media

低强度聚焦超声对急性中耳炎小鼠模型中耳的影响

• 发表时间: 2013
• 期刊: Ultrasound Med Biol
• 影响因子: 2.9
• 作者: Noda K;Hirano T;Noda K;Kodama S;Ichimiya I;Suzuki M
• 通讯作者: Suzuki M

Effect of phosphorycholine on mucin production and gene expression in the middle ear in the mice (poster)

磷酸胆碱对小鼠中耳粘蛋白产生和基因表达的影响（海报）

• 发表时间: 2011
• 作者: Iwasaki T.;Hirano T.;Kodama S.;Suzuki M
• 通讯作者: Suzuki M

肺炎球菌-特集 ワクチン-

肺炎球菌-特色疫苗-

• 发表时间: 2012
• 期刊: 耳喉頭頸
• 作者: Noda Ka.;Hirano T.;Noda Ke.;Kodama S.;Ichimiya I.;Suzuki M.;山中敏彰;平野 隆
• 通讯作者: 平野 隆

Effect of regulartory T cells on bacterial clearance in vitro assay

常规 T 细胞对体外细菌清除的影响

• 发表时间: 2011
• 期刊: Recent Advances in Otitis media, in press
• 作者: Hirano T.;Kodama S.;Kawano T.;Suzuki M
• 通讯作者: Suzuki M

Pili play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarrhalis

在卡他莫拉菌急性中耳炎小鼠模型中，菌毛在增强中耳细菌清除方面发挥着重要作用

• DOI: 10.1111/2049-632x.12025
• 发表时间: 2013
• 期刊: pathogens and disease
• 影响因子: 3.3
• 作者: Kawano T.,Hirano T.,Kodama S.;Mitsui MT.,Ahmed K.,Nishizono A.;Suzuki M
• 通讯作者: Suzuki M