Analysis of progress of grades in urinary bladder cancer : telomere measurement with original software and chromosomal instability

膀胱癌分级进展分析：原版软件端粒测量与染色体不稳定性

• 批准号: 22590327
• 负责人: NAKAMURA Kenichi
• 金额: $ 2.83万
• 依托单位: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590327/
• 关键词: 膀胱癌; テロメア; FISH; 染色体不安定性; アナフェースブリッジ; Q-FISH

In order to evaluate the relationships existing among three histologic types of urothelial tumors, chromosomal instability, and telomere length, we examined 37 consecutive cases of papillary urothelial neoplasm, from which we obtained 26 (70.3%) that were suitable for karyotype analysis, comprising 7 PUNLMP, 10 low-grade papillary urothelial carcinomas (PUCs), and 9 high-grade PUCs. We subjected these cases to karyotype and anaphase bridge analyses, and measured their telomere lengths by Q-FISH. PUNLMP always showed diploidy and had anaphase bridges. Low-grade PUC showed diploidy (n = 2), hypoploidy (n = 4) and polyploidy (n = 4), and high-grade PUC revealed diploidy (n = 1) and polyploidy (n = 8); both had anaphase bridges. The incidence of anaphase bridges did not differ significantly between PUNLMP and high-grade PUC (p = 0.105). The telomere lengths of PUNLMP, low-grade PUC, and high-grade PUC, expressed as mean telomere fluorescence units (TFU) ± SD, were 7906 ± 3197, 4893 ± 1567, and 3299 ± 1406, respectively. The differences among the three groups were significant. However, 42.9% of PUNLMPs had shorter telomeres than the mean value for low-grade PUC, and 30.0% of the low-grade PUCs had shorter telomeres than those for high-grade PUC. There was an inverse correlation between telomere length and the incidence of anaphase bridges. It is suggested that PUNLMP progresses to low- and high-grade PUC in association with telomere shortening and chromosomal instability. Our data support the idea that critically shortened telomeres cause chromosomal instability during progression of papillary urothelial neoplasms..

为了评估尿路上皮肿瘤的三种组织学类型之间的关系，染色体不稳定性检查了37例乳头状肿瘤病例，我们从中获得了26（70.3％），适合于核型分析，这些分析可升高7个PUNLMP，10个低级乳头乳房乳腺癌（ PUC）和9个高级PUC。高级PUC揭示了二倍体（n = 1）8）;荧光单位（TFU）±SD分别为7906±3197，4893±1406，这三组差异很大。比高度桥梁短。 PAP LILARY尿路上皮肿瘤..

项目成果

東京都健康長寿医療センター研究所（東京都老人総合研究所）老年病理学研究チーム高齢者がん研究グループ

东京都老年学中心研究所（东京都老年学研究所）老年病理学研究组、老年癌症研究组

Q-FISH法による膀胱癌の悪性度とテロメア長

Q-FISH法分析膀胱癌的恶性程度和端粒长度

• 发表时间: 2011
• 作者: 仲村賢一;他
• 通讯作者: 他