Biological function and gene regulation of epididymal-specific carboxylesterase (CES4)

附睾特异性羧酸酯酶（CES4）的生物学功能和基因调控

• 批准号: 22580100
• 负责人: YAMASHITA Tetsuro
• 金额: $ 3万
• 依托单位: Iwate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580100/
• 关键词: 精巣上体; エステラーゼ; 酵素; カルボキシルエステラーゼ; 遺伝子変異マウス; 巣上体; KOマウス; 精巣上体; エステラーゼ; 酵素; カルボキシルエステラーゼ; 遺伝子変異マウス; 巣上体; KOマウス

The biological function of an epididymal-specific carboxylesterase, CES4, was studied using Ces4 knock out mouse. The metabolome analysis of epididymal cell extracts revealed that the most of the glutathione was identified as oxidative form in the epididymis of KO mouse. The expression of oxidative stress-responsive heme oxygenase-1 (HO-1) was also increased in the KO mouse tissue. These data indicated that the epididymis of KO mouse was exposed by oxidative stress.

使用CES4敲除小鼠研究了附睾特异性羧酸酯酶CES4的生物学功能。附子细胞提取物的代谢组分析表明，大部分谷胱甘肽被确定为KO小鼠附睾中的氧化形式。在KO小鼠组织中，氧化应激响应性血红素氧酶-1（HO-1）的表达也增加了。这些数据表明，KO小鼠的附睾是通过氧化应激暴露的。