Integrated reaction system based on W/O emulsion in microchannel

基于微通道W/O乳液的集成反应系统

• 批准号: 15360440
• 负责人: KATSURA Shinji
• 金额: $ 9.98万
• 依托单位: Toyohashi University of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15360440/
• 关键词: Microchannel; Microcreator; Electrostatic operation; Droplet; Miniaturization; Micropump; PCR; W; Oエマルション; 静電霧化現象; EHD; 水滴

In this research project, we have been engaged in development of micro-reactor system based on droplets handling in continuous oil phase. In this system, those droplets function as extremely miniaturized reactors. In order to establish handling technique of the micro-reactors, we investigated methods to production and division of droplets, and also electric micro-pump system to handle droplets in micro-channel.First of all, we examined droplet formation by electro-spray, and succeeded in division of droplets by applying AC high voltage to miniaturized electrodes. Furthermore, we developed a micro-pump system based on synergetic effects of laser irradiation and AC electric field application. Because this pump lacks movable part, it can be miniaturized easily.As an important application of emulsified reactors, we applied water-in-oil emulsion to PCR. This emulsified PCR permitted amplification of both single molecule DNA and RNA. However, when emulsification is carried out by stirring, it is hard to exclude possibility of contamination. Especially single molecule PCR, contamination is a serious problem. We also developed a new emulsification system based on electric field application. In this system, we succeeded in emulsification by applying electric field by electrodes outside of tubes. This method can reduce possibility of contamination and permit application to W/O emulsion system for single molecule PCR.

在这个研究项目中，我们致力于开发基于连续油相液滴处理的微反应器系统。在这个系统中，这些液滴充当极其小型化的反应器。为了建立微反应器的处理技术，我们研究了液滴的产生和分割方法，以及在微通道中处理液滴的电动微泵系统。并通过向微型电极施加交流高压，成功地分割了液滴。此外，我们还开发了一种基于激光照射和交流电场应用协同效应的微型泵系统。由于该泵没有可动部件，因此易于小型化。作为乳化反应器的一个重要应用，我们将油包水乳液应用于PCR。这种乳化 PCR 可以扩增单分子 DNA 和 RNA。然而，当通过搅拌进行乳化时，很难排除污染的可能性。尤其是单分子PCR，污染是一个严重的问题。我们还开发了一种基于电场应用的新型乳化系统。在该系统中，我们通过管外电极施加电场成功地实现了乳化。该方法可以减少污染的可能性，并允许应用于单分子 PCR 的 W/O 乳液系统。

项目成果

Electrostatic droplet-formation in water/oil flow in a microchannel system

微通道系统中水/油流中的静电液滴形成

• 发表时间: 2004
• 作者: Nakano M.; Nakai N.; Inoue M.; Takashima K.; Katsura S.; Nuzuno A.
• 通讯作者: Nuzuno A.

Single-Molecule Reverse Transcription Polymerase Chain Reaction Using Water-In-Oil emulsion

使用油包水乳液的单分子逆转录聚合酶链反应

• 作者: NAKANO M.; NAKAI N.; KURITA H.; KOMATSU J.; TAKASHIMA K.; KATSURA S.; MIZUNO A.
• 通讯作者: MIZUNO A.

Estimation of apparent AC transport losses in cylindrical conductors composed of superconductor tapes

由超导带组成的圆柱形导体中表观交流传输损耗的估计

• 发表时间: 2005
• 作者: R.Inada; Y.Nakamura; A.Oota; T.Fukunaga
• 通讯作者: T.Fukunaga

Electrostatic droplet-formation in water/oil flow in a microchannel system

微通道系统中水/油流中的静电液滴形成

• 发表时间: 2004
• 作者: Nakano M.; Nakai N.; Inoue M.; Takashima K.; Katsura S.; Mizuno A.
• 通讯作者: Mizuno A.

Stretching of T4 and Lambda DNA molecule by flow induced by laser and AC voltage

通过激光和交流电压诱导的流动拉伸 T4 和 Lambda DNA 分子

• 发表时间: 2005
• 作者: Michihiko Nakano; Shinji Katsura; Akira Mizuno
• 通讯作者: Akira Mizuno