Production with high quality nitrogen fixing legume plants by SNARE

SNARE 采用优质固氮豆科植物进行生产

• 批准号: 22580067
• 负责人: AGARIE Mika
• 金额: $ 2.91万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580067/
• 关键词: ミヤコグサ; SNARE; 根粒菌; 共生; 窒素固定; 根粒; GUS; 毛状根形質転換; RNAi; RT-PCR; Fix-; ミヤコグサ; SNARE; 根粒菌; 共生; 窒素固定; 根粒; GUS; 毛状根形質転換; RNAi; RT-PCR; Fix-

Exocytosis and endocytosis are fundamental in plant development, homeostasis and interaction with the environment. These are highly dynamic processed that, even in otherwise static plant tissue, engage a rapid turnover of large areas of membrane surface. SNAREs (soluble N-ethyl maleimide sensitive factor attachment protein receptors) proteins drive membrane and protein targeting and delivery in eukaryotic cells. Since higher plants have complicated and multiple membrane traffic systems, the membrane fusion system would be important for plant development and signal transduction. We have screening SNARE genes which interact with nodule development. The suppression of Syn1, Syn37, Gen06 genes by the RNAi could form nodule but the most of the nodules were white in the hairly root nodule. When we infected LacZ-labeled M.loti, blue stained M.loti could see at the nodule. GFP fused Syn1, Syn37, Gen06 proteins revealed that these proteins were located on the plasma membrane or endosome in the Arabidopsis cultured cell. These data suggest that Syn1, Syn37, Gen06 SNARE play a vital role in the turnover of integral membrane proteins in signaling and (or) nutrition in the nodule.

胞吞作用和内吞作用是植物发育，稳态和与环境相互作用的基础。这些是高度动态的处理，即使在否则静态植物组织中，也会迅速营业的膜表面。网罗（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）蛋白质驱动膜和蛋白质的靶向和真核细胞的递送。由于较高的植物具有复杂和多个膜交通系统，因此膜融合系统对于植物发育和信号转导至关重要。我们有与结节发育相互作用的筛选基因。 RNAi抑制Syn1，Syn37，Gen06基因可以形成结节，但大部分结节在毛状根结节中是白色的。当我们感染了LacZ标记的M.loti时，蓝色染色的M.loti可以在结节上看到。 GFP Fused Syn1，Syn37，Gen06蛋白显示，这些蛋白质位于质膜上或拟南芥培养细胞中的内体上。这些数据表明SYN1，SYN37，GEN06 SNARE在整体膜蛋白在信号传导中和（或）结节中的营养中起着至关重要的作用。