Development of a quantitative and non-traumatic strategy to insert any nano-molecules into living cells with electrospray

开发定量和非创伤性策略，通过电喷雾将任何纳米分子插入活细胞中

• 批准号: 22500302
• 负责人: VIGOT Rejan
• 金额: $ 2.91万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22500302/
• 关键词: Electrospray; Transfection; Neuron; 電子スプレー; 遺伝子導入; 神経細胞; ナノバイオ; 脳神経疾患; 先端機能デバイス; Molecular and cellular neuroscience; Molecular and cellular neurobiology; Neuron, synapse, and neural circuit; Neural plasticity; Neurotransmitters and receptors

We designed a nanoelectrospray micronozzle to ionize, accelerate and focus molecules/particles toward a single neuron for injection. The two concentric capillaries with hydrophobic coating produced an electrospray of solutions, with localized deposition on solid target. Precise nozzle alignment and voltage wave form were both critical to achieve stable droplet production with peak current reaching 50-250nA. With anionic dye and epifluorescence microscopy, we observed particles reaching the gas-medium interface but also run-away droplets accumulation on the inside of the outer pipette, until an abrupt flooding of the tip and leak of solution. Various nozzle morphologies were tested but this limitation is intrinsic to the current nozzle. Improved designs based on simulations of electrostatic properties are presented.

我们设计了一种纳米电喷嘴，以电离，加速和聚焦分子/颗粒向单个神经元进行注射。两个带有疏水涂层的同心毛细血管产生了溶液的电喷雾，并在固体靶标上进行了局部沉积。精确的喷嘴比对和电压波形对于实现稳定的液滴产生至关重要，峰值电流达到50-250NA。使用阴离子染料和落叶显微镜，我们观察到达到气体界面的颗粒，但滴水液滴在外移液的内部积聚，直到尖端突然泛滥和溶液的泄漏。测试了各种喷嘴形态，但是这种限制是当前喷嘴的固有。提出了基于静电性能的模拟的改进设计。